| Literature DB >> 3377794 |
J C Sacchettini1, M W Frazier, D C Chiara, L J Banaszak, G A Grant.
Abstract
The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.Entities:
Mesh：
Substances：
Year: 1988 PMID： 3377794 DOI： 10.1016/s0006-291x(88)81243-9
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575