Gabriella-Salome K Armstrong1, Jacqueline A Frank1,2, Christopher J McLouth3, Ann Stowe1,4,2, Jill M Roberts1,2, Amanda L Trout1,2, Justin F Fraser1,5,6,4,2, Keith Pennypacker1,4,2. 1. Department of Neurology, University of Kentucky, Lexington, Kentucky, USA. 2. Center for Advanced Translational Stroke Science, University of Kentucky, Lexington, Kentucky, USA. 3. Department of Behavioral Science, University of Kentucky, Lexington, Kentucky, USA. 4. Department of Neuroscience, University of Kentucky, Lexington, Kentucky, USA. 5. Department of Neurosurgery, University of Kentucky, Lexington, Kentucky, USA. 6. Department of Radiology, University of Kentucky, Lexington, Kentucky, USA.
Abstract
INTRODUCTION: Uromodulin (UMOD) is a glycoprotein expressed by the epithelial cells of the thick ascending limb of Henle's loop in the kidney. Research has shown that increased uromodulin expression may be associated with lower risk of cardiovascular disease in adults. Utilizing the Blood and Clot Thrombectomy Registry and Collaboration (BACTRAC) (clinicaltrials.gov NCT03153683), a continuously enrolling tissue bank, we aimed to examine the associations between serum uromodulin, age, and high BMI (BMI>25) and its relationship to stroke in patients. METHODS: Arterial blood distal and proximal to the thrombus was collected during a thrombectomy procedure using the BACTRAC protocol and sent to Olink (Boston, MA) to determine proteomic expression via proximity extension assay. Uromodulin expression was recorded and analyzed using two tailed T-tests and linear regressions. RESULTS: The relationship between systemic and intracranial uromodulin, age, high BMI and hypertension were assessed. Systemic and intracranial uromodulin decreased with age (p<0.0001 and r2=0.343, p=0.0416 and r2=0.102) respectively. Systemic uromodulin expression increased with BMI>25 (p=0.014). Presence of hypertension decreased uromodulin's expression systemically (p=0.018) and intracranially (p=0.007). CONCLUSIONS: Uromodulin was increased significantly in overweight patients, decreased significantly in older patients, and decreased in patients with hypertension. The increase in uromodulin in people with high BMI could be a protective reaction of the kidney to worsening conditions that make ischemic stroke more likely, with a goal of delaying dangerous outcomes. The decreased expression of uromodulin in older adults could be associated with the decline of general kidney function that accompanies aging. Hypertension can contribute to an AKI by decreasing perfusion to the kidney, therefore decreasing kidney function and uromodulin production. Further analyses are needed to understand the role of uromodulin following ischemic stroke.
INTRODUCTION: Uromodulin (UMOD) is a glycoprotein expressed by the epithelial cells of the thick ascending limb of Henle's loop in the kidney. Research has shown that increased uromodulin expression may be associated with lower risk of cardiovascular disease in adults. Utilizing the Blood and Clot Thrombectomy Registry and Collaboration (BACTRAC) (clinicaltrials.gov NCT03153683), a continuously enrolling tissue bank, we aimed to examine the associations between serum uromodulin, age, and high BMI (BMI>25) and its relationship to stroke in patients. METHODS: Arterial blood distal and proximal to the thrombus was collected during a thrombectomy procedure using the BACTRAC protocol and sent to Olink (Boston, MA) to determine proteomic expression via proximity extension assay. Uromodulin expression was recorded and analyzed using two tailed T-tests and linear regressions. RESULTS: The relationship between systemic and intracranial uromodulin, age, high BMI and hypertension were assessed. Systemic and intracranial uromodulin decreased with age (p<0.0001 and r2=0.343, p=0.0416 and r2=0.102) respectively. Systemic uromodulin expression increased with BMI>25 (p=0.014). Presence of hypertension decreased uromodulin's expression systemically (p=0.018) and intracranially (p=0.007). CONCLUSIONS: Uromodulin was increased significantly in overweight patients, decreased significantly in older patients, and decreased in patients with hypertension. The increase in uromodulin in people with high BMI could be a protective reaction of the kidney to worsening conditions that make ischemic stroke more likely, with a goal of delaying dangerous outcomes. The decreased expression of uromodulin in older adults could be associated with the decline of general kidney function that accompanies aging. Hypertension can contribute to an AKI by decreasing perfusion to the kidney, therefore decreasing kidney function and uromodulin production. Further analyses are needed to understand the role of uromodulin following ischemic stroke.
Entities:
Keywords:
Age; Blood and Clot Thrombectomy Registry and Collaboration (BACTRAC); High Body Mass Index (BMI); Ischemic Stroke; Uromodulin protein
Authors: Sandosh Padmanabhan; Lesley Graham; Nicholas R Ferreri; Delyth Graham; Martin McBride; Anna F Dominiczak Journal: Hypertension Date: 2014-08-04 Impact factor: 10.190
Authors: Justin F Fraser; Lisa A Collier; Amy A Gorman; Sarah R Martha; Kathleen E Salmeron; Amanda L Trout; Danielle N Edwards; Stephanie M Davis; Douglas E Lukins; Abdulnasser Alhajeri; Stephen Grupke; Jill M Roberts; Gregory J Bix; Keith R Pennypacker Journal: J Neurointerv Surg Date: 2018-07-31 Impact factor: 5.836
Authors: Graciela E Delgado; Marcus E Kleber; Hubert Scharnagl; Bernhard K Krämer; Winfried März; Jürgen E Scherberich Journal: J Am Soc Nephrol Date: 2017-02-27 Impact factor: 10.121
Authors: Benton Maglinger; Jacqueline A Frank; Christopher J McLouth; Amanda L Trout; Jill Marie Roberts; Stephen Grupke; Jadwiga Turchan-Cholewo; Ann M Stowe; Justin F Fraser; Keith R Pennypacker Journal: J Neurointerv Surg Date: 2020-07-08 Impact factor: 5.836