| Literature DB >> 33765312 |
Brunella Posteraro1, Flavio De Maio2, Antonio Gasbarrini3.
Abstract
In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.Entities:
Keywords: 16S ribosomal DNA; Microbial community; Next-generation sequencing; PCR amplification; Sequence-based analysis; Stool samples; Targeted amplicon sequencing
Mesh：
Substances：
Year: 2021 PMID： 33765312 DOI： 10.1007/978-1-0716-1302-3_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745