Nathan Blanke1, Veronica Go2, Douglas L Rosene2, Irving J Bigio1,3. 1. Boston University, Neurophotonics Center, Department of Biomedical Engineering, Boston, Massachusetts, United States. 2. Boston University School of Medicine, Department of Anatomy and Neurobiology, Boston, Massachusetts, United States. 3. Boston University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United States.
Abstract
Significance: Myelin breakdown is likely a key factor in the loss of cognitive and motor function associated with many neurodegenerative diseases. Aim: New methods for imaging myelin structure are needed to characterize and quantify the degradation of myelin in standard whole-brain sections of nonhuman primates and in human brain. Approach: Quantitative birefringence microscopy (qBRM) is a label-free technique for rapid histopathological assessment of myelin structural breakdown following cortical injury in rhesus monkeys. Results: We validate birefringence microscopy for structural imaging of myelin in rhesus monkey brain sections, and we demonstrate the power of qBRM by characterizing the breakdown of myelin following cortical injury, as a model of stroke, in the motor cortex. Conclusions: Birefringence microscopy is a valuable tool for histopathology of myelin and for quantitative assessment of myelin structure. Compared to conventional methods, this label-free technique is sensitive to subtle changes in myelin structure, is fast, and enables more quantitative assessment, without the variability inherent in labeling procedures such as immunohistochemistry.
Significance: Myelin breakdown is likely a key factor in the loss of cognitive and motor function associated with many neurodegenerative diseases. Aim: New methods for imaging myelin structure are needed to characterize and quantify the degradation of myelin in standard whole-brain sections of nonhuman primates and in human brain. Approach: Quantitative birefringence microscopy (qBRM) is a label-free technique for rapid histopathological assessment of myelin structural breakdown following cortical injury in rhesus monkeys. Results: We validate birefringence microscopy for structural imaging of myelin in rhesus monkey brain sections, and we demonstrate the power of qBRM by characterizing the breakdown of myelin following cortical injury, as a model of stroke, in the motor cortex. Conclusions: Birefringence microscopy is a valuable tool for histopathology of myelin and for quantitative assessment of myelin structure. Compared to conventional methods, this label-free technique is sensitive to subtle changes in myelin structure, is fast, and enables more quantitative assessment, without the variability inherent in labeling procedures such as immunohistochemistry.
Authors: Ezzat Hashemi; Ezra Yoseph; Hsing-Chuan Tsai; Monica Moreno; Li-Hao Yeh; Shalin B Mehta; Mari Kono; Richard Proia; May H Han Journal: Cell Mol Neurobiol Date: 2022-08-02 Impact factor: 4.231
Authors: Chao J Liu; William Ammon; Robert J Jones; Jackson Nolan; Ruopeng Wang; Shuaibin Chang; Matthew P Frosch; Anastasia Yendiki; David A Boas; Caroline Magnain; Bruce Fischl; Hui Wang Journal: Biomed Opt Express Date: 2021-12-15 Impact factor: 3.562