Pia U Olafson1, Serap Aksoy2, Geoffrey M Attardo3, Greta Buckmeier4, Xiaoting Chen5, Craig J Coates6, Megan Davis4, Justin Dykema7, Scott J Emrich8, Markus Friedrich7, Christopher J Holmes9, Panagiotis Ioannidis10, Evan N Jansen9, Emily C Jennings9, Daniel Lawson11, Ellen O Martinson12, Gareth L Maslen11, Richard P Meisel13, Terence D Murphy14, Dana Nayduch15, David R Nelson16, Kennan J Oyen9, Tyler J Raszick6, José M C Ribeiro17, Hugh M Robertson18, Andrew J Rosendale19, Timothy B Sackton20, Perot Saelao4, Sonja L Swiger21, Sing-Hoi Sze22, Aaron M Tarone6, David B Taylor23, Wesley C Warren24, Robert M Waterhouse25, Matthew T Weirauch26,27, John H Werren28, Richard K Wilson29,30, Evgeny M Zdobnov10, Joshua B Benoit31. 1. Livestock Arthropod Pests Research Unit, USDA-ARS, Kerrville, TX, USA. pia.olafson@usda.gov. 2. Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA. 3. Department of Entomology and Nematology, University of California - Davis, Davis, CA, USA. 4. Livestock Arthropod Pests Research Unit, USDA-ARS, Kerrville, TX, USA. 5. The Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. 6. Department of Entomology, Texas A & M University, College Station, TX, USA. 7. Department of Biological Sciences, Wayne State University, Detroit, MI, USA. 8. Department of Electrical Engineering & Computer Science, University of Tennessee, Knoxville, TN, USA. 9. Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA. 10. Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, 1211, Geneva, Switzerland. 11. The European Molecular Biology Laboratory, The European Bioinformatics Institute, The Wellcome Genome Campus, Hinxton, CB10 1SD, UK. 12. Department of Entomology, University of Georgia, Athens, GA, USA. 13. Department of Biology and Biochemistry, University of Houston, Houston, TX, USA. 14. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA. 15. Arthropod-borne Animal Diseases Research Unit, USDA-ARS, Manhattan, KS, USA. 16. Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, USA. 17. Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Rockville, MD, USA. 18. Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL, USA. 19. Department of Biology, Mount St. Joseph University, Cincinnati, OH, USA. 20. Informatics Group, Faculty of Arts and Sciences, Harvard University, Cambridge, MA, USA. 21. Department of Entomology, Texas A&M AgriLife Research and Extension Center, Stephenville, TX, USA. 22. Department of Computer Science & Engineering, Department of Biochemistry & Biophysics, Texas A & M University, College Station, TX, USA. 23. Agroecosystem Management Research Unit, USDA-ARS, Lincoln, NE, USA. 24. University of Missouri, Bond Life Sciences Center, Columbia, MO, USA. 25. Department of Ecology and Evolution, University of Lausanne, and Swiss Institute of Bioinformatics, 1015, Lausanne, Switzerland. 26. Center for Autoimmune Genomics and Etiology, Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. 27. Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA. 28. Department of Biology, University of Rochester, Rochester, NY, USA. 29. Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA. 30. College of Medicine, Ohio State University, Columbus, OH, USA. 31. Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA. joshua.benoit@uc.edu.
Abstract
BACKGROUND: The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. RESULTS: This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. CONCLUSIONS: The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha.
BACKGROUND: The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. RESULTS: This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. CONCLUSIONS: The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha.
Authors: Taciany Ferreira de Souza Dominghetti; Antonio Thadeu Medeiros de Barros; Cleber Oliveira Soares; Paulo Henrique Duarte Cançado Journal: Rev Bras Parasitol Vet Date: 2015 Oct-Dec
Authors: M A Birkett; N Agelopoulos; K-M V Jensen; J B Jespersen; J A Pickett; H J Prijs; G Thomas; J J Trapman; L J Wadhams; C M Woodcock Journal: Med Vet Entomol Date: 2004-12 Impact factor: 2.739
Authors: D Traversa; D Otranto; R Iorio; A Carluccio; A Contri; B Paoletti; R Bartolini; A Giangaspero Journal: Med Vet Entomol Date: 2008-09 Impact factor: 2.739
Authors: Andrew D Yates; James Allen; Ridwan M Amode; Andrey G Azov; Matthieu Barba; Andrés Becerra; Jyothish Bhai; Lahcen I Campbell; Manuel Carbajo Martinez; Marc Chakiachvili; Kapeel Chougule; Mikkel Christensen; Bruno Contreras-Moreira; Alayne Cuzick; Luca Da Rin Fioretto; Paul Davis; Nishadi H De Silva; Stavros Diamantakis; Sarah Dyer; Justin Elser; Carla V Filippi; Astrid Gall; Dionysios Grigoriadis; Cristina Guijarro-Clarke; Parul Gupta; Kim E Hammond-Kosack; Kevin L Howe; Pankaj Jaiswal; Vinay Kaikala; Vivek Kumar; Sunita Kumari; Nick Langridge; Tuan Le; Manuel Luypaert; Gareth L Maslen; Thomas Maurel; Benjamin Moore; Matthieu Muffato; Aleena Mushtaq; Guy Naamati; Sushma Naithani; Andrew Olson; Anne Parker; Michael Paulini; Helder Pedro; Emily Perry; Justin Preece; Mark Quinton-Tulloch; Faye Rodgers; Marc Rosello; Magali Ruffier; James Seager; Vasily Sitnik; Michal Szpak; John Tate; Marcela K Tello-Ruiz; Stephen J Trevanion; Martin Urban; Doreen Ware; Sharon Wei; Gary Williams; Andrea Winterbottom; Magdalena Zarowiecki; Robert D Finn; Paul Flicek Journal: Nucleic Acids Res Date: 2022-01-07 Impact factor: 16.971
Authors: Pia U Olafson; Serap Aksoy; Geoffrey M Attardo; Greta Buckmeier; Xiaoting Chen; Craig J Coates; Megan Davis; Justin Dykema; Scott J Emrich; Markus Friedrich; Christopher J Holmes; Panagiotis Ioannidis; Evan N Jansen; Emily C Jennings; Daniel Lawson; Ellen O Martinson; Gareth L Maslen; Richard P Meisel; Terence D Murphy; Dana Nayduch; David R Nelson; Kennan J Oyen; Tyler J Raszick; José M C Ribeiro; Hugh M Robertson; Andrew J Rosendale; Timothy B Sackton; Perot Saelao; Sonja L Swiger; Sing-Hoi Sze; Aaron M Tarone; David B Taylor; Wesley C Warren; Robert M Waterhouse; Matthew T Weirauch; John H Werren; Richard K Wilson; Evgeny M Zdobnov; Joshua B Benoit Journal: BMC Biol Date: 2021-07-29 Impact factor: 7.431