Mauricio Garrido1, Diego Morales2,3,4, María Paz Saldías2,3,4, Christian Fernández2,3,4, Veronica Villalobos2,3,4, Oscar Cerda2,3,4, Mónica Cáceres5,6,7. 1. Department of Conservative Dentistry, Faculty of Dentistry, Universidad de Chile, Santiago, Chile. 2. Program of Molecular and Cell Biology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile. 3. Millenium Nucleus of Ion Channel-Associated Diseases (MiNICAD), Santiago, Chile. 4. Faculty of Medicine, Universidad de Chile, Santiago, Chile. 5. Program of Molecular and Cell Biology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile. monicacaceres@med.uchile.cl. 6. Millenium Nucleus of Ion Channel-Associated Diseases (MiNICAD), Santiago, Chile. monicacaceres@med.uchile.cl. 7. Faculty of Medicine, Universidad de Chile, Santiago, Chile. monicacaceres@med.uchile.cl.
Abstract
BACKGROUND: This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials. METHODS: Primary cells were obtained from explants of young immature premolars. 20,000 cells adhered for 24 h over discs of Biodentine™, ProRoot®MTA, BioRoot®RCS and calcium hydroxide mixed either with sodium chloride 0.9%w/v or polyethylene glycol and UltraCal® were used to evaluate cell adhesion by scanning electron microscopy and cell viability by MTT assay. RESULTS: Cells adhered to ProRoot®MTA showed an increase of F-actin like protrusions, suggesting bioactivity. Cells adhered to UltraCal® show protrusion such as filopodia. On the contrary, cells adhered to BioRoot®RCS showed no signs of any cellular protrusion. Regarding viability between the materials, we found a higher percentage of viability in cells cultured over discs of Biodentine™ and ProRoot®MTA. CONCLUSION: ProRoot®MTA and Biodentine™ exhibit a better cellular response of human apical papilla cells in vitro conditions compared to BioRoot® and calcium hydroxide diluted in sodium chloride.
BACKGROUND: This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials. METHODS: Primary cells were obtained from explants of young immature premolars. 20,000 cells adhered for 24 h over discs of Biodentine™, ProRoot®MTA, BioRoot®RCS and calcium hydroxide mixed either with sodium chloride 0.9%w/v or polyethylene glycol and UltraCal® were used to evaluate cell adhesion by scanning electron microscopy and cell viability by MTT assay. RESULTS: Cells adhered to ProRoot®MTA showed an increase of F-actin like protrusions, suggesting bioactivity. Cells adhered to UltraCal® show protrusion such as filopodia. On the contrary, cells adhered to BioRoot®RCS showed no signs of any cellular protrusion. Regarding viability between the materials, we found a higher percentage of viability in cells cultured over discs of Biodentine™ and ProRoot®MTA. CONCLUSION: ProRoot®MTA and Biodentine™ exhibit a better cellular response of human apical papilla cells in vitro conditions compared to BioRoot® and calcium hydroxide diluted in sodium chloride.
Authors: E J N L Silva; T Accorsi-Mendonça; J F A Almeida; C C R Ferraz; B P F A Gomes; A A Zaia Journal: Int Endod J Date: 2011-09-13 Impact factor: 5.264
Authors: M Brito-Júnior; R D Pereira; C Veríssimo; C J Soares; A L Faria-e-Silva; C C Camilo; M D Sousa-Neto Journal: Int Endod J Date: 2014-02-01 Impact factor: 5.264
Authors: María Paz Saldías; Christian Fernández; Alejandra Morgan; Catalina Díaz; Diego Morales; Fabián Jaña; Alvaro Gómez; Alonso Silva; Fernanda Briceño; Alejandro Oyarzún; Felipe Maldonado; Oscar Cerda; Patricio C Smith; Mónica Cáceres Journal: PLoS One Date: 2017-09-12 Impact factor: 3.240