Yuan-Bo Pan1, Jianan Lu1, Biao Yang2, Cameron Lenahan3,4, Jianmin Zhang5,6,7, Anwen Shao8. 1. Department of Neurosurgery, School of Medicine, Second Affiliated Hospital, Zhejiang University, NO.88 Jiefang Rd, Hangzhou, 310009, Zhejiang, China. 2. Department of Neurosurgery, Huashan Hospital of Fudan University, Shanghai, China. 3. Burrell College of Osteopathic Medicine, Las Cruces, NM, 88003, USA. 4. Center for Neuroscience Research, School of Medicine, Loma Linda University, Loma Linda, CA, 92324, USA. 5. Department of Neurosurgery, School of Medicine, Second Affiliated Hospital, Zhejiang University, NO.88 Jiefang Rd, Hangzhou, 310009, Zhejiang, China. zjm135@zju.edu.cn. 6. Brain Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. zjm135@zju.edu.cn. 7. Collaborative Innovation Center for Brain Science, Zhejiang University, Hangzhou, Zhejiang, China. zjm135@zju.edu.cn. 8. Department of Neurosurgery, School of Medicine, Second Affiliated Hospital, Zhejiang University, NO.88 Jiefang Rd, Hangzhou, 310009, Zhejiang, China. 2316040@zju.edu.cn.
Abstract
BACKGROUND: Rupture of intracranial aneurysm (IA) is the main cause of devastating subarachnoid hemorrhage, which urges our understanding of the pathogenesis and regulatory mechanisms of IA. However, the regulatory roles of long non-coding RNAs (lncRNAs) in IA is less known. RESULTS: We processed the raw SRR files of 12 superficial temporal artery (STA) samples and 6 IA samples to count files. Then the differentially expressed (DE) mRNAs, miRNAs, and lncRNAs between STAs and IAs were identified. The enrichment analyses were performed using DEmRNAs. Next, a lncRNA-miRNA-mRNA regulatory network was constructed using integrated bioinformatics analysis. In summary, 341 DElncRNAs, 234 DEmiRNAs, and 2914 DEmRNAs between the STA and IA. The lncRNA-miRNA-mRNA regulatory network of IA contains 91 nodes and 146 edges. The subnetwork of hub lncRNA PVT1 was extracted. The expression level of PVT1 was positively correlated with a majority of the mRNAs in its subnetwork. Moreover, we found that several mRNAs (CCND1, HIF1A, E2F1, CDKN1A, VEGFA, COL1A1 and COL5A2) in the PVT1 subnetwork served as essential components in the PI3K-Akt signaling pathway, and that some of the non-coding RNAs (ncRNAs) (PVT1, HOTAIR, hsa-miR-17, hsa-miR-142, hsa-miR-383 and hsa-miR-193b) interacted with these mRNAs. CONCLUSION: Our annotations noting ncRNA's role in the pathway may uncover novel regulatory mechanisms of ncRNAs and mRNAs in IA. These findings provide significant insights into the lncRNA regulatory network in IA.
BACKGROUND: Rupture of intracranial aneurysm (IA) is the main cause of devastating subarachnoid hemorrhage, which urges our understanding of the pathogenesis and regulatory mechanisms of IA. However, the regulatory roles of long non-coding RNAs (lncRNAs) in IA is less known. RESULTS: We processed the raw SRR files of 12 superficial temporal artery (STA) samples and 6 IA samples to count files. Then the differentially expressed (DE) mRNAs, miRNAs, and lncRNAs between STAs and IAs were identified. The enrichment analyses were performed using DEmRNAs. Next, a lncRNA-miRNA-mRNA regulatory network was constructed using integrated bioinformatics analysis. In summary, 341 DElncRNAs, 234 DEmiRNAs, and 2914 DEmRNAs between the STA and IA. The lncRNA-miRNA-mRNA regulatory network of IA contains 91 nodes and 146 edges. The subnetwork of hub lncRNA PVT1 was extracted. The expression level of PVT1 was positively correlated with a majority of the mRNAs in its subnetwork. Moreover, we found that several mRNAs (CCND1, HIF1A, E2F1, CDKN1A, VEGFA, COL1A1 and COL5A2) in the PVT1 subnetwork served as essential components in the PI3K-Akt signaling pathway, and that some of the non-coding RNAs (ncRNAs) (PVT1, HOTAIR, hsa-miR-17, hsa-miR-142, hsa-miR-383 and hsa-miR-193b) interacted with these mRNAs. CONCLUSION: Our annotations noting ncRNA's role in the pathway may uncover novel regulatory mechanisms of ncRNAs and mRNAs in IA. These findings provide significant insights into the lncRNA regulatory network in IA.
Authors: Kimon Bekelis; Joanna S Kerley-Hamilton; Amy Teegarden; Craig R Tomlinson; Rachael Kuintzle; Nathan Simmons; Robert J Singer; David W Roberts; Manolis Kellis; David A Hendrix Journal: J Neurosurg Date: 2016-02-26 Impact factor: 5.115
Authors: Mitja I Kurki; Sanna-Kaisa Häkkinen; Juhana Frösen; Riikka Tulamo; Mikael von und zu Fraunberg; Garry Wong; Gerard Tromp; Mika Niemelä; Juha Hernesniemi; Juha E Jääskeläinen; Seppo Ylä-Herttuala Journal: Neurosurgery Date: 2011-06 Impact factor: 4.654
Authors: Henriëtte E Westerlaan; J M C van Dijk; M J van Dijk; Marijke C Jansen-van der Weide; Jan Cees de Groot; Rob J M Groen; Jan Jakob A Mooij; Matthijs Oudkerk Journal: Radiology Date: 2010-10-08 Impact factor: 11.105