Yang Hong1, Jiahui Si1, Jie Zhang2, Ying Xiong1, Jianzhi Zhang1, Peter Ping Lin3, Jian Fang2, Yue Yang1, Chao Lv1, Yuanyuan Ma1. 1. Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China. 2. Department of Thoracic Oncology II, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China. 3. Department of Oncology, Cytelligen, San Diego, CA, United States.
Abstract
OBJECTIVE: The size distribution of circulating aneuploid cells (CACs) and its clinical significance were investigated in resectable non-small cell lung cancer (NSCLC). PATIENTS AND METHODS: A total of 50 patients with resectable NSCLC were enrolled in this study. Blood samples (50 pre-surgery and 35 post-surgery) were collected and used for the detection of CAC chromosome 8 heteroploidy through the subtraction enrichment and immunostaining fluorescence in situ hybridization (SE-iFISH) method. RESULTS: Less than 20% small cell size and more than 80% large cell size CACs were detected. Karyotypes, including triploid, tetraploid, and multiploid, had varying distributions. The triploid subtype accounted for the majority of small cell size CACs, whereas the multiploid subtype accounted for the majority of large cell size CACs. We found that total small cell size and triploid small cell size CACs, but not large cell size CACs, derived from pre-surgery samples, were associated with shorter disease-free survival. Moreover, total small cell size and triploid small cell size CACs were associated with higher TNM stage and recurrence. Nevertheless, the variation between pre- and post-surgery CACs was not related to survival among patients with resectable NSCLC. CONCLUSIONS: Pre-surgery small cell size CACs, especially the triploid subtype, could be regarded as a potential prognostic biomarker for patients with resectable NSCLC.
OBJECTIVE: The size distribution of circulating aneuploid cells (CACs) and its clinical significance were investigated in resectable non-small cell lung cancer (NSCLC). PATIENTS AND METHODS: A total of 50 patients with resectable NSCLC were enrolled in this study. Blood samples (50 pre-surgery and 35 post-surgery) were collected and used for the detection of CAC chromosome 8 heteroploidy through the subtraction enrichment and immunostaining fluorescence in situ hybridization (SE-iFISH) method. RESULTS: Less than 20% small cell size and more than 80% large cell size CACs were detected. Karyotypes, including triploid, tetraploid, and multiploid, had varying distributions. The triploid subtype accounted for the majority of small cell size CACs, whereas the multiploid subtype accounted for the majority of large cell size CACs. We found that total small cell size and triploid small cell size CACs, but not large cell size CACs, derived from pre-surgery samples, were associated with shorter disease-free survival. Moreover, total small cell size and triploid small cell size CACs were associated with higher TNM stage and recurrence. Nevertheless, the variation between pre- and post-surgery CACs was not related to survival among patients with resectable NSCLC. CONCLUSIONS: Pre-surgery small cell size CACs, especially the triploid subtype, could be regarded as a potential prognostic biomarker for patients with resectable NSCLC.
Authors: Virginia Arrazubi; Elena Mata; María Luisa Antelo; Antonio Tarifa; Javier Herrera; Cruz Zazpe; Lucía Teijeira; Antonio Viudez; Javier Suárez; Irene Hernández; Ruth Vera Journal: Ann Surg Oncol Date: 2019-06-17 Impact factor: 5.344
Authors: Maria T Sandri; Laura Zorzino; Maria C Cassatella; Fabio Bassi; Alberto Luini; Chiara Casadio; Edoardo Botteri; Nicole Rotmensz; Laura Adamoli; Franco Nolè Journal: Ann Surg Oncol Date: 2010-02-05 Impact factor: 5.344
Authors: Ida Netterberg; Mats O Karlsson; Leon W M M Terstappen; Miriam Koopman; Cornelis J A Punt; Lena E Friberg Journal: Clin Cancer Res Date: 2020-06-11 Impact factor: 12.531