| Literature DB >> 33742406 |
Philip Dujardin1, Barbara M Grüner2.
Abstract
DNA barcoding allows the quantitative, biomarker-free tracking of individual cell populations in mixed/heterogeneous cell pools. Here, we describe a multiplexed in vivo screening platform based on DNA barcoding technology to interrogate compound libraries for their effect on metastatic seeding in vivo. We apply next-generation sequencing (NGS) technology to quantitatively analyze high-throughput compound screening in mice. Up to 96 compounds and controls can be screened for their effect on metastatic ability in a single mouse.Entities:
Keywords: Chemical compound screens; DNA barcoding; In vivo screening; Metastasis; Mouse transplantation models; Next-generation sequencing; Retroviral vector transduction
Mesh：
Year: 2021 PMID： 33742406 DOI： 10.1007/978-1-0716-1350-4_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745