Literature DB >> 33742391

The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells.

Jörg H Leupold1,2, Nitin Patil1,2, Heike Allgayer3,4.   

Abstract

The CAM model enables an in vivo analysis of the individual sub-steps of the metastatic cascade like local invasion, intravasation, or the establishment of metastasis in particular organs. Incubated fertilized chicken eggs are inoculated with human tumor cells and further processed for up to 9-10 days. The invasion and metastasis of these cells is then detected quantitatively with high specificity and sensitivity by means of a PCR for human ALU sequences, using the genomic DNA isolated from distant portions of the CAM, as well as from diverse internal organs of the developing embryo.

Entities:  

Keywords:  ALU elements; Chicken chorioallantoic membrane; DNA; In vivo model; Metastasis; Primer; Probe; Quantification; Real-time PCR; TaqMan

Mesh：

Year:  2021        PMID： 33742391     DOI： 10.1007/978-1-0716-1350-4_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction.

Authors:  Andries Zijlstra; Rebecca Mellor; Giano Panzarella; Ronald T Aimes; John D Hooper; Natalia D Marchenko; James P Quigley
Journal:  Cancer Res       Date:  2002-12-01       Impact factor: 12.701

2.  Immunological capacity of the chicken embryo. I. Relationship between the maturation of lymphoid tissues and the occurrence of cell-mediated immunity in the developing chicken embryo.

Authors:  B D Janković; K Isaković; M L Lukić; N L Vujanović; S Petrović; B M Marković
Journal:  Immunology       Date:  1975-09       Impact factor: 7.397

3.  Growth of transplantable human tumors in the chick embryo and hatched chick.

Authors:  C P DAGG; D A KARNOFSKY; J RODDY
Journal:  Cancer Res       Date:  1956-08       Impact factor: 12.701

4.  TaqMan-based quantification of invasive cells in the chick embryo metastasis assay.

Authors:  Edward Htun van der Horst; Jörg H Leupold; Rainer Schubbert; Axel Ullrich; Heike Allgayer
Journal:  Biotechniques       Date:  2004-12       Impact factor: 1.993

5.  A human-specific subfamily of Alu sequences.

Authors:  M A Batzer; P L Deininger
Journal:  Genomics       Date:  1991-03       Impact factor: 5.736

6.  Ontogeny and function of two non-lymphoid cell populations in the chicken embryo.

Authors:  E M Janse; S H Jeurissen
Journal:  Immunobiology       Date:  1991-08       Impact factor: 3.144

7.  Tumor suppressor Pdcd4 inhibits invasion/intravasation and regulates urokinase receptor (u-PAR) gene expression via Sp-transcription factors.

Authors:  J H Leupold; H-S Yang; N H Colburn; I Asangani; S Post; H Allgayer
Journal:  Oncogene       Date:  2007-02-12       Impact factor: 9.867

8.  Development of bioluminescent chick chorioallantoic membrane (CAM) models for primary pancreatic cancer cells: a platform for drug testing.

Authors:  Maria Rovithi; Amir Avan; Niccola Funel; Leticia G Leon; Valentina E Gomez; Thomas Wurdinger; Arjan W Griffioen; Henk M W Verheul; Elisa Giovannetti
Journal:  Sci Rep       Date:  2017-03-17       Impact factor: 4.379

9.  Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis.

Authors:  Noor A Lokman; Alison S F Elder; Carmela Ricciardelli; Martin K Oehler
Journal:  Int J Mol Sci       Date:  2012-08-10       Impact factor: 6.208

10.  TRANSPLANTABILITY OF TISSUES TO THE EMBRYO OF FOREIGN SPECIES : ITS BEARING ON QUESTIONS OF TISSUE SPECIFICITY AND TUMOR IMMUNITY.

Authors:  J B Murphy
Journal:  J Exp Med       Date:  1913-04-01       Impact factor: 14.307
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