Hein M Tun1, Ye Peng2, Bolin Chen3, Theodore B Konya4, Nadia P Morales-Lizcano4, Radha Chari5, Catherine J Field6, David S Guttman7, Allan B Becker8, Piush J Mandhane3, Theo J Moraes9, Malcolm R Sears10, Stuart E Turvey11, Padmaja Subbarao9, Elinor Simons8, James A Scott4, Anita L Kozyrskyj12. 1. HKU-Pasteur Research Pole, School of Public Health, University of Hong Kong, Hong Kong, China; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada. Electronic address: heinmtun@hku.hk. 2. HKU-Pasteur Research Pole, School of Public Health, University of Hong Kong, Hong Kong, China. 3. Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada. 4. Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada. 5. Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada. 6. Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Alberta, Canada. 7. Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada. 8. Department of Pediatrics and Child Health, Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada. 9. Department of Pediatrics, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada. 10. Department of Medicine, McMaster University, Hamilton, ON, Canada. 11. Department of Pediatrics, Child and Family Research Institute, BC Children's Hospital, University of British Columbia, Vancouver, British Columbia, Canada. 12. Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada. Electronic address: kozyrsky@ualberta.ca.
Abstract
BACKGROUND AND AIMS: Increasing evidence supports the role of early-life gut microbiota in developing atopic diseases, but ecological changes to gut microbiota during infancy in relation to food sensitization remain unclear. We aimed to characterize and associate these changes with the development of food sensitization in children. METHODS: In this observational study, using 16S rRNA amplicon sequencing, we characterized the composition of 2844 fecal microbiota in 1422 Canadian full-term infants. Atopic sensitization outcomes were measured by skin prick tests at age 1 year and 3 years. The association between gut microbiota trajectories, based on longitudinal shifts in community clusters, and atopic sensitization outcomes at age 1 and 3 years were determined. Ethnicity and early-life exposures influencing microbiota trajectories were initially examined, and post hoc analyses were conducted. RESULTS: Four identified developmental trajectories of gut microbiota were shaped by birth mode and varied by ethnicity. The trajectory with persistently low Bacteroides abundance and high Enterobacteriaceae/Bacteroidaceae ratio throughout infancy increased the risk of sensitization to food allergens, particularly to peanuts at age 3 years by 3-fold (adjusted odds ratio [OR] 2.82, 95% confidence interval [CI] 1.13-7.01). A much higher likelihood for peanut sensitization was found if infants with this trajectory were born to Asian mothers (adjusted OR 7.87, 95% CI 2.75-22.55). It was characterized by a deficiency in sphingolipid metabolism and persistent Clostridioides difficile colonization. Importantly, this trajectory of depleted Bacteroides abundance mediated the association between Asian ethnicity and food sensitization. CONCLUSIONS: This study documented an association between persistently low gut Bacteroides abundance throughout infancy and sensitization to peanuts in childhood. It is the first to show a mediation role for infant gut microbiota in ethnicity-associated development of food sensitization.
BACKGROUND AND AIMS: Increasing evidence supports the role of early-life gut microbiota in developing atopic diseases, but ecological changes to gut microbiota during infancy in relation to food sensitization remain unclear. We aimed to characterize and associate these changes with the development of food sensitization in children. METHODS: In this observational study, using 16S rRNA amplicon sequencing, we characterized the composition of 2844 fecal microbiota in 1422 Canadian full-term infants. Atopic sensitization outcomes were measured by skin prick tests at age 1 year and 3 years. The association between gut microbiota trajectories, based on longitudinal shifts in community clusters, and atopic sensitization outcomes at age 1 and 3 years were determined. Ethnicity and early-life exposures influencing microbiota trajectories were initially examined, and post hoc analyses were conducted. RESULTS: Four identified developmental trajectories of gut microbiota were shaped by birth mode and varied by ethnicity. The trajectory with persistently low Bacteroides abundance and high Enterobacteriaceae/Bacteroidaceae ratio throughout infancy increased the risk of sensitization to food allergens, particularly to peanuts at age 3 years by 3-fold (adjusted odds ratio [OR] 2.82, 95% confidence interval [CI] 1.13-7.01). A much higher likelihood for peanut sensitization was found if infants with this trajectory were born to Asian mothers (adjusted OR 7.87, 95% CI 2.75-22.55). It was characterized by a deficiency in sphingolipid metabolism and persistent Clostridioides difficile colonization. Importantly, this trajectory of depleted Bacteroides abundance mediated the association between Asian ethnicity and food sensitization. CONCLUSIONS: This study documented an association between persistently low gut Bacteroides abundance throughout infancy and sensitization to peanuts in childhood. It is the first to show a mediation role for infant gut microbiota in ethnicity-associated development of food sensitization.
Authors: Rachael G Horne; Stephen B Freedman; Kathene C Johnson-Henry; Xiao-Li Pang; Bonita E Lee; Ken J Farion; Serge Gouin; Suzanne Schuh; Naveen Poonai; Katrina F Hurley; Yaron Finkelstein; Jianling Xie; Sarah Williamson-Urquhart; Linda Chui; Laura Rossi; Michael G Surette; Philip M Sherman Journal: Front Cell Infect Microbiol Date: 2022-06-14 Impact factor: 6.073
Authors: Siew C Ng; Ye Peng; Lin Zhang; Chris Kp Mok; Shilin Zhao; Amy Li; Jessica Yl Ching; Yingzhi Liu; Shuai Yan; Dream L S Chan; Jie Zhu; Chunke Chen; Adrian Ch Fung; Kenneth Ky Wong; David Sc Hui; Francis Kl Chan; Hein M Tun Journal: Gut Date: 2022-02-09 Impact factor: 31.793
Authors: Roosa Jokela; Katri Korpela; Ching Jian; Evgenia Dikareva; Anne Nikkonen; Terhi Saisto; Kirsi Skogberg; Willem M de Vos; Kaija-Leena Kolho; Anne Salonen Journal: Gut Microbes Date: 2022 Jan-Dec
Authors: Christophe Lay; Collins Wenhan Chu; Rikky Wenang Purbojati; Enzo Acerbi; Daniela I Drautz-Moses; Paola Florez de Sessions; Song Jie; Eliza Ho; Yee Jiun Kok; Xuezhi Bi; Shuwen Chen; Shi Ya Mak; Mei Chien Chua; Anne E N Goh; Wen Chin Chiang; Rajeshwar Rao; Surasith Chaithongwongwatthana; Nipon Khemapech; Voranush Chongsrisawat; Rocio Martin; Guus Roeselers; Ying Swan Ho; Martin L Hibberd; Stephan C Schuster; Jan Knol Journal: BMC Microbiol Date: 2021-06-25 Impact factor: 3.605