Murat Gurger1, Gokhan Once2, Erhan Yilmaz1, Sukru Demir1, Ilknur Calik3, Yakup Say4, Ahmet Kavakli5, Sefa Key1, Mustafa Umit Gurbuz1, Onur Bingollu1. 1. Department of Orthopaedics and Traumatology, Faculty of Medicine, Firat University, 23190, Elazig, Turkey. 2. Department of Orthopaedics and Traumatology, Faculty of Medicine, Firat University, 23190, Elazig, Turkey. gokhanonce2334@gmail.com. 3. Department of Medical Pathology, Faculty of Medicine, Firat University, 23190, Elazig, Turkey. 4. Department of Metallurgical and Materials Engineering, Tunceli University, 62000, Tunceli, Turkey. 5. Department of Anatomy, Faculty of Medicine, Firat University, 23190, Elazig, Turkey.
Abstract
BACKGROUND: The aim of this study is to histologically and biomechanically investigate the effects of local PRP and ozone therapy (O2O3) on tendon-to-bone healing in a rabbit model of the supraspinatus tendon tear. METHODS: Four groups were formed to have seven rabbits in each group: repair, R; repair + PRP, RP; repair + ozone, RO; and repair + PRP + ozone, RPO. The supraspinatus tendon was detached by sharp dissection from the footprint and an acute tear pattern was created. Thereafter, tendon repair was performed with the transosseous technique. In the RP group, PRP, and in the RPO group, PRP + O2O3 mixture was injected to the tendon repair site. In the RO group, O2O3 gas mixture was injected into subacromial space three times a week for a total of 4 weeks. The study was ended at postoperative 6th week. RESULTS: When compared with the R group, a statistically significant increase was observed in the biomechanical strength of the RP and RPO groups. The highest increase in biomechanical strength was detected in the RPO group. The histology of the RO and RPO groups showed better collagen fiber continuity and orientation than the R and RP groups. CONCLUSIONS: The results obtained from this study show that the ozonized PRP can be used as biological support to increase tendon-to-bone healing. However, these results need to be supported by clinical studies.
BACKGROUND: The aim of this study is to histologically and biomechanically investigate the effects of local PRP and ozone therapy (O2O3) on tendon-to-bone healing in a rabbit model of the supraspinatus tendon tear. METHODS: Four groups were formed to have seven rabbits in each group: repair, R; repair + PRP, RP; repair + ozone, RO; and repair + PRP + ozone, RPO. The supraspinatus tendon was detached by sharp dissection from the footprint and an acute tear pattern was created. Thereafter, tendon repair was performed with the transosseous technique. In the RP group, PRP, and in the RPO group, PRP + O2O3 mixture was injected to the tendon repair site. In the RO group, O2O3 gas mixture was injected into subacromial space three times a week for a total of 4 weeks. The study was ended at postoperative 6th week. RESULTS: When compared with the R group, a statistically significant increase was observed in the biomechanical strength of the RP and RPO groups. The highest increase in biomechanical strength was detected in the RPO group. The histology of the RO and RPO groups showed better collagen fiber continuity and orientation than the R and RP groups. CONCLUSIONS: The results obtained from this study show that the ozonized PRP can be used as biological support to increase tendon-to-bone healing. However, these results need to be supported by clinical studies.
Authors: Matthew B Murphy; Daniel Blashki; Rachel M Buchanan; Iman K Yazdi; Mauro Ferrari; Paul J Simmons; Ennio Tasciotti Journal: Biomaterials Date: 2012-04-28 Impact factor: 12.479
Authors: Francisco Javier Hidalgo-Tallón; Luis Miguel Torres-Morera; Jose Baeza-Noci; Maria Dolores Carrillo-Izquierdo; Rosa Pinto-Bonilla Journal: Front Physiol Date: 2022-02-23 Impact factor: 4.566