Claire E O'Leary1, Xiaogang Feng2, Victor S Cortez1, Richard M Locksley1,3,4, Christoph Schneider2. 1. Department of Medicine, University of California, San Francisco, San Francisco, California. 2. Institute of Physiology, University of Zurich, Zurich, Switzerland. 3. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California. 4. Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California.
Abstract
Recent findings position tuft cells as key mediators of intestinal immunity through their production of the cytokine interleukin (IL)-25 and activation of group 2 innate lymphoid cells (ILC2s). Though tuft cells are found in numerous epithelial tissues, their phenotype and function have been best characterized in the small intestine, where robust in vivo techniques have enabled the dissection of their cellular function, ontogeny, and key signaling pathways. We describe methods for the identification, quantification, and manipulation of tuft cells, focusing on analysis of ILC2s as a readout of tuft cell function.
Recent findings position tuft cells as key mediators of intestinal immunity through their production of the cytokine interleukin (IL)-25 and activation of group 2 innate lymphoid cells (ILC2s). Though tuft cells are found in numerous epithelial tissues, their phenotype and function have been best characterized in the small intestine, where robust in vivo techniques have enabled the dissection of their cellular function, ontogeny, and key signaling pathways. We describe methods for the identification, quantification, and manipulation of tuft cells, focusing on analysis of ILC2s as a readout of tuft cell function.
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