Daniel Woods1, Danielle S LeSassier2, Ikechukwu Egbunam3, Christopher W Lennon4. 1. Wadsworth Center, New York State Department of Health, Albany, New York. 2. Signature Science, LLC, Austin, Texas. 3. Department of Biology, University at Albany, Albany, New York. 4. Department of Biology, Murray State University, Murray, Kentucky.
Abstract
Inteins (intervening proteins) are translated within host proteins and removed through protein splicing. Conditional protein splicing (CPS), where the rate and accuracy of splicing are highly dependent on environmental cues, has emerged as a novel form of post-translational regulation. While CPS has been demonstrated for several inteins in vitro, a comprehensive understanding of inteins requires tools to quantitatively monitor their activity within the cellular context. Here, we describe a method for construction of a splicing-dependent system that can be used to quantitatively assay for conditions that modulate protein splicing.
Inteins (intervening proteins) are translated within host proteins and removed through protein splicing. Conditional protein splicing (CPS), where the rate and accuracy of splicing are highly dependent on environmental cues, has emerged as a novel form of post-translational regulation. While CPS has been demonstrated for several inteins in vitro, a comprehensive understanding of inteins requires tools to quantitatively monitor their activity within the cellular context. Here, we describe a method for construction of a splicing-dependent system that can be used to quantitatively assay for conditions that modulate protein splicing.
Authors: Julie N Reitter; Christopher E Cousin; Michael C Nicastri; Mario V Jaramillo; Kenneth V Mills Journal: Biochemistry Date: 2016-02-26 Impact factor: 3.162