Patrícia Molz1,2,3, Betânia Souza de Freitas2, Vanise Hallas Uberti2, Kesiane Mayra da Costa1,3, Luiza Wilges Kist1, Maurício Reis Bogo1,3,4, Nadja Schröder5,6. 1. Laboratory of Genomics and Molecular Biology, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. 2. Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. 3. Graduate Program in Medicine and Health Sciences, School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. 4. National Institute of Science and Technology for Brain Diseases, Excitotoxicity and Neuroprotection (INCT-EN), Porto Alegre, Brazil. 5. National Institute of Science and Technology for Translational Medicine (INCT-TM), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasília, Brazil. nadja_s@terra.com.br. 6. Department of Physiology, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, Brazil. nadja_s@terra.com.br.
Abstract
PURPOSE: To investigate the effects of lipoic acid (LA) supplementation during adulthood combined with supplementation later in life or LA administration only at old age on age-induced cognitive dysfunction, mitochondrial DNA deletions, caspase 3 and antioxidant response enzymes expression in iron-treated rats. METHODS: Male rats were submitted to iron treatment (30 mg/kg body wt of Carbonyl iron) from 12 to 14th post-natal days. Iron-treated rats received LA supplementation (50 mg/kg, daily) in adulthood and old age or at old age only for 21 days. Memory, mitochondrial DNA (mtDNA) complex I deletions, caspase 3 mRNA expression and antioxidant response enzymes mRNA expression were analyzed in the hippocampus. RESULTS: LA administration in adulthood combined with treatment later in life was able to reverse age-induced effects on object recognition and inhibitory avoidance memory, as well as on mtDNA deletions, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression, and antioxidant enzymes disruption induced by iron in aged rats. LA treatment only at old age reversed iron-induced effects to a lesser extent when compared to the combined treatment. CONCLUSION: The present findings support the view that LA supplementation may be considered as an adjuvant against mitochondrial damage and cognitive decline related to aging and neurodegenerative disorders.
PURPOSE: To investigate the effects of lipoic acid (LA) supplementation during adulthood combined with supplementation later in life or LA administration only at old age on age-induced cognitive dysfunction, mitochondrial DNA deletions, caspase 3 and antioxidant response enzymes expression in iron-treated rats. METHODS: Male rats were submitted to iron treatment (30 mg/kg body wt of Carbonyl iron) from 12 to 14th post-natal days. Iron-treated rats received LA supplementation (50 mg/kg, daily) in adulthood and old age or at old age only for 21 days. Memory, mitochondrial DNA (mtDNA) complex I deletions, caspase 3 mRNA expression and antioxidant response enzymes mRNA expression were analyzed in the hippocampus. RESULTS: LA administration in adulthood combined with treatment later in life was able to reverse age-induced effects on object recognition and inhibitory avoidance memory, as well as on mtDNA deletions, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression, and antioxidant enzymes disruption induced by iron in aged rats. LA treatment only at old age reversed iron-induced effects to a lesser extent when compared to the combined treatment. CONCLUSION: The present findings support the view that LA supplementation may be considered as an adjuvant against mitochondrial damage and cognitive decline related to aging and neurodegenerative disorders.
Authors: Maria Noemia M de Lima; Manuela Polydoro; Daniela C Laranja; Fernanda Bonatto; Elke Bromberg; José Cláudio F Moreira; Felipe Dal-Pizzol; Nadja Schröder Journal: Eur J Neurosci Date: 2005-05 Impact factor: 3.386
Authors: M N de Lima; J Presti-Torres; V A Garcia; M R Guimarães; F S Scalco; R Roesler; N Schröder Journal: Neuropharmacology Date: 2008-06-21 Impact factor: 5.250
Authors: Vanessa Kappel da Silva; Betânia Souza de Freitas; Arethuza da Silva Dornelles; Laura Roesler Nery; Lucio Falavigna; Rafael Dal Ponte Ferreira; Maurício Reis Bogo; Jaime Eduardo Cecílio Hallak; Antônio Waldo Zuardi; José Alexandre S Crippa; Nadja Schröder Journal: Mol Neurobiol Date: 2013-07-28 Impact factor: 5.590