Literature DB >> 33732800

Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva.

Raffaella Klima1, Giulia Romano1, Monsurat Gbadamosi1, Aram Megighian2,3, Fabian Feiguin1.   

Abstract

Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through immune-electrophysiology in Drosophila melanogaster. This technique allows the quantification of the molecular behavior of the neuromuscular synapses by correlating the structural configuration of the synaptic boutons with their electrical activity.
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Drosophila melanogaster; Electrophysiology; Immunocytochemistry; Neurological disorders; Neuromuscular synapses; Synaptic boutons; Third instar larvae

Year:  2021        PMID： 33732800      PMCID： PMC7953129          DOI： 10.21769/BioProtoc.3913

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  12 in total

1.  Arg206 of SNAP-25 is essential for neuroexocytosis at the Drosophila melanogaster neuromuscular junction.

Authors:  Aram Megighian; Michele Scorzeto; Damiano Zanini; Sergio Pantano; Michela Rigoni; Clara Benna; Ornella Rossetto; Cesare Montecucco; Mauro Zordan
Journal:  J Cell Sci       Date:  2010-09-07       Impact factor: 5.285

2.  Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and in grasshopper embryos.

Authors:  L Y Jan; Y N Jan
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

3.  Chronological requirements of TDP-43 function in synaptic organization and locomotive control.

Authors:  Giulia Romano; Raffaella Klima; Emanuele Buratti; Patrik Verstreken; Francisco E Baralle; Fabian Feiguin
Journal:  Neurobiol Dis       Date:  2014-08-01       Impact factor: 5.996

4.  Depletion of TDP-43 affects Drosophila motoneurons terminal synapsis and locomotive behavior.

Authors:  Fabian Feiguin; Vinay K Godena; Giulia Romano; Andrea D'Ambrogio; Raffaella Klima; Francisco E Baralle
Journal:  FEBS Lett       Date:  2009-04-19       Impact factor: 4.124

5.  Improved stability of Drosophila larval neuromuscular preparations in haemolymph-like physiological solutions.

Authors:  B A Stewart; H L Atwood; J J Renger; J Wang; C F Wu
Journal:  J Comp Physiol A       Date:  1994-08       Impact factor: 1.836

6.  Estimation of the Readily Releasable Synaptic Vesicle Pool at the Drosophila Larval Neuromuscular Junction.

Authors:  Pragya Goel; Xiling Li; Dion Dickman
Journal:  Bio Protoc       Date:  2019-01-05

7.  FM1-43 Photoconversion and Electron Microscopy Analysis at the Drosophila Neuromuscular Junction.

Authors:  Nadezhda S Sabeva; Maria Bykhovskaia
Journal:  Bio Protoc       Date:  2017-09-05

8.  TDP-43 regulates Drosophila neuromuscular junctions growth by modulating Futsch/MAP1B levels and synaptic microtubules organization.

Authors:  Vinay K Godena; Giulia Romano; Maurizio Romano; Chiara Appocher; Raffaella Klima; Emanuele Buratti; Francisco E Baralle; Fabian Feiguin
Journal:  PLoS One       Date:  2011-03-11       Impact factor: 3.240

9.  Glial TDP-43 regulates axon wrapping, GluRIIA clustering and fly motility by autonomous and non-autonomous mechanisms.

Authors:  Giulia Romano; Chiara Appocher; Michele Scorzeto; Raffaella Klima; Francisco E Baralle; Aram Megighian; Fabian Feiguin
Journal:  Hum Mol Genet       Date:  2015-08-13       Impact factor: 6.150

10.  TDP-43 promotes the formation of neuromuscular synapses through the regulation of Disc-large expression in Drosophila skeletal muscles.

Authors:  Nina Strah; Giulia Romano; Clelia Introna; Raffaella Klima; Marta Marzullo; Laura Ciapponi; Aram Megighian; Monica Nizzardo; Fabian Feiguin
Journal:  BMC Biol       Date:  2020-03-26       Impact factor: 7.431
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