Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Induction of Epithelial-mesenchymal Transition in MDCK II Cells.

Literature DB >> 33732790

Induction of Epithelial-mesenchymal Transition in MDCK II Cells.

Abstract

Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into a mesenchymal cell, that enables initiation of cell migration. EMT plays an important role in embryonic development, tissue repair and cancer metastasis. Better understanding of cellular and molecular events during EMT will not only provide novel insights on how mammalian organism develops and how epithelial tissues regenerate, but also can identify novel therapeutic targets for cancer therapy. Here we aim to provide a detailed protocol on how to induce EMT in Madin-Darby Canine Kidney (MDCK) II epithelial cell line and perform immunofluorescent staining on EMT-induced cells.

Entities: Chemical

Keywords: EMT; Epithelial plasticity; Epithelial-mesenchymal transition; Hepatocyte growth factor; MDCK cells; Mesenchymal cell differentiation from epithelial cell

Year: 2021 PMID： 33732790 PMCID： PMC7953130 DOI： 10.21769/BioProtoc.3903

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

Keyword Cloud
References

11 in total

1. HGF induces epithelial-to-mesenchymal transition by modulating the mammalian hippo/MST2 and ISG15 pathways.

Authors: Jennifer Farrell; Ciara Kelly; Jens Rauch; Katarzyna Kida; Amaya García-Muñoz; Naser Monsefi; Benedetta Turriziani; Carolanne Doherty; Jai P Mehta; David Matallanas; Jeremy C Simpson; Walter Kolch; Alex von Kriegsheim
Journal: J Proteome Res Date: 2014-05-05 Impact factor: 4.466

2. IL-6 promotes head and neck tumor metastasis by inducing epithelial-mesenchymal transition via the JAK-STAT3-SNAIL signaling pathway.

Authors: Arti Yadav; Bhavna Kumar; Jharna Datta; Theodoros N Teknos; Pawan Kumar
Journal: Mol Cancer Res Date: 2011-10-05 Impact factor: 5.852

Review 3. Cellular Plasticity in Cancer.

Authors: Salina Yuan; Robert J Norgard; Ben Z Stanger
Journal: Cancer Discov Date: 2019-04-16 Impact factor: 39.397

4. Epithelial-mesenchymal transition induced by TNF-α requires NF-κB-mediated transcriptional upregulation of Twist1.

Authors: Chia-Wei Li; Weiya Xia; Longfei Huo; Seung-Oe Lim; Yun Wu; Jennifer L Hsu; Chi-Hong Chao; Hirohito Yamaguchi; Neng-Kai Yang; Qingqing Ding; Yan Wang; Yun-Ju Lai; Adam M LaBaff; Ting-Jung Wu; Been-Ren Lin; Muh-Hwa Yang; Gabriel N Hortobagyi; Mien-Chie Hung
Journal: Cancer Res Date: 2012-01-17 Impact factor: 12.701

Review 5. The basics of epithelial-mesenchymal transition.

Authors: Raghu Kalluri; Robert A Weinberg
Journal: J Clin Invest Date: 2009-06 Impact factor: 14.808

Review 6. The MDCK variety pack: choosing the right strain.

Authors: Joseph D Dukes; Paul Whitley; Andrew D Chalmers
Journal: BMC Cell Biol Date: 2011-10-07 Impact factor: 4.241

7. RANKL promotes migration and invasion of hepatocellular carcinoma cells via NF-κB-mediated epithelial-mesenchymal transition.

Authors: Fang-Nan Song; Meng Duan; Long-Zi Liu; Zhi-Chao Wang; Jie-Yi Shi; Liu-Xiao Yang; Jian Zhou; Jia Fan; Qiang Gao; Xiao-Ying Wang
Journal: PLoS One Date: 2014-09-30 Impact factor: 3.240