Literature DB >> 33732756

Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts.

Rituraj Batth1, Ilenuta Simina Cuciurean1, Satish Kumar Kodiripaka1, Sarah Skovlund Rothman1, Caroline Greisen1, Henrik Toft Simonsen1.   

Abstract

This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with enzyme cocktails mix of 1.5% Cellulase ONOZUKA R10 and 0.5% Macerozyme R10 to generate 1,8 million protoplasts within 3 h.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Cellulase; Macerozyme; Moss; Physcomitrium patens (syn. Physcomitrella patens) ; Protoplastation

Year:  2021        PMID： 33732756      PMCID： PMC7952925          DOI： 10.21769/BioProtoc.3782

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

1.  Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.

Authors:  Sang-Dong Yoo; Young-Hee Cho; Jen Sheen
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

2.  Chemical and UV mutagenesis of spores and protonemal tissue from the moss Physcomitrella patens.

Authors:  David J Cove; Pierre-François Perroud; Audra J Charron; Stuart F McDaniel; Abha Khandelwal; Ralph S Quatrano
Journal:  Cold Spring Harb Protoc       Date:  2009-02

3.  Heterologous stable expression of terpenoid biosynthetic genes using the moss Physcomitrella patens.

Authors:  Søren Spanner Bach; Brian Christopher King; Xin Zhan; Henrik Toft Simonsen; Björn Hamberger
Journal:  Methods Mol Biol       Date:  2014

4.  Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression.

Authors:  S Abel; A Theologis
Journal:  Plant J       Date:  1994-03       Impact factor: 6.417

Review 5.  Physcomitrella patens, a versatile synthetic biology chassis.

Authors:  Ralf Reski; Hansol Bae; Henrik Toft Simonsen
Journal:  Plant Cell Rep       Date:  2018-05-24       Impact factor: 4.570

6.  The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation.

Authors:  Igor Fesenko; Anna Seredina; Georgij Arapidi; Vasily Ptushenko; Anatoly Urban; Ivan Butenko; Sergey Kovalchuk; Konstantin Babalyan; Andrey Knyazev; Regina Khazigaleeva; Elena Pushkova; Nikolai Anikanov; Vadim Ivanov; Vadim M Govorun
Journal:  Front Plant Sci       Date:  2016-11-04       Impact factor: 5.753

7.  Perspectives on using physcomitrella patens as an alternative production platform for thapsigargin and other terpenoid drug candidates.

Authors:  Henrik Toft Simonsen; Damian Paul Drew; Christina Lunde
Journal:  Perspect Medicin Chem       Date:  2009-03-04

8.  In vivo assembly of DNA-fragments in the moss, Physcomitrella patens.

Authors:  Brian Christopher King; Konstantinos Vavitsas; Nur Kusaira Binti Khairul Ikram; Josephine Schrøder; Lars B Scharff; Jean-Étienne Bassard; Björn Hamberger; Poul Erik Jensen; Henrik Toft Simonsen
Journal:  Sci Rep       Date:  2016-04-29       Impact factor: 4.379

9.  Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.).

Authors:  S V G N Priyadarshani; Bingyan Hu; Weimin Li; Hina Ali; Haifeng Jia; Lihua Zhao; Simon Peter Ojolo; Syed Muhammad Azam; Junjie Xiong; Maokai Yan; Zia Ur Rahman; Qingsong Wu; Yuan Qin
Journal:  Plant Methods       Date:  2018-10-29       Impact factor: 4.993
  9 in total
  1 in total

Review 1.  Plant cell cultures as heterologous bio-factories for secondary metabolite production.

Authors:  Tong Wu; Sandra M Kerbler; Alisdair R Fernie; Youjun Zhang
Journal:  Plant Commun       Date:  2021-08-23
  1 in total

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