Synthesis of Silicon Nanoparticles Emitting Yellow-Green Fluorescence for Visualization of pH Change and Determination of Intracellular pH of Living Cells.

In order to understand related pathogenesis of some diseases and design new intracellular drug delivery systems, investigation of pH change in living cells in real time is important. In this paper, a new style of fluorescent silicon nanoparticles (SiNPs) as a pH-sensitive probe and for the visualization of the pH changes in cells was designed and prepared using 4-aminophenol as a reducing agent and N-aminoethyl-γ-aminopropyltrimethyl as a silicon source by a one-pot hydrothermal method. It was particularly noteworthy that the fluorescence intensity emitted from the SiNPs positively correlated with the pH value of solutions, making the SiNPs a viable probe used for sensitive sensing of pH. At the same time, a response of the probe to the pH was found in 5.0-10.0, and the SiNPs have an excellent biocompatibility (e.g., ∼74% of cell viability was remained after treatment for 24 h at 500 μg/mL of the SiNPs). The proposed method that could display the change in pH of live cells provided an effective means for visually diagnosing diseases related to intracellular pH.