The Use of Novel Non-Vitamin K Antagonist Oral Anticoagulants Following Closure of the Left Atrial Appendage: Preliminary Results of Clinical Follow-Up.

OBJECTIVE: The aim of this study was to investigate the safety and efficacy of using novel non-vitamin K antagonist oral anticoagulants (NOACs) for anticoagulation following left atrial appendage closure (LAAC).
METHODS: A total of 70 patients with non-valvular atrial fibrillation (NVAF) were enrolled in this study. All patients underwent successful LAAC between November 2018 and September 2019 in the Department of Cardiology of the First Affiliated Hospital of the University of Science and Technology of China. All patients subsequently completed a 45-day postoperative follow-up period. Patients were grouped according to the postoperative anticoagulation regimen they received: there were 40 patients in the NOACs group and 30 patients in the warfarin group. Baseline clinical data, intraoperative data, and short-term postoperative follow-up data were collected and the two groups were compared.
RESULTS: The intraoperative results showed no statistical difference between the two groups in respect of the occlusion rate, the compression ratio of the occluder, the volume of pericardial effusion, or the incidence of the following: residual shunts, device-related thromboses (DRT), stroke, and pericardial effusion (P > 0.05 in all cases). The residual shunt volume in the NOACs group was significantly smaller than that in the warfarin group (P = 0.04). During the 45-day postoperative follow-up period, there was no statistical difference between the two groups in respect of the residual shunt volume, or the incidence of DRT, ischemic stroke and cerebral hemorrhage (P > 0.05 in all cases). Compared with the warfarin group, the residual shunt volume was significantly reduced in the NOACs group (P = 0.03). The incidence of minor hemorrhage and the total hemorrhage incidence in the NOACs group were significantly lower than those in the warfarin group (5% vs 30%, P = 0.004, and 5% vs 33.3%, P = 0.002, respectively).
CONCLUSION: In this study, the use of NOACs for postoperative anticoagulation therapy following LAAC did not increase the risk of embolization or hemorrhage during the short-term follow-up period.

Introduction

Non-valvular atrial fibrillation (NVAF) is a common clinical arrhythmia, and stroke and thromboembolic events are common and serious complications in patients with this condition. The incidence of stroke and thromboembolic events is two to seven times higher than in patients without atrial fibrillation (AF). In patients with AF, ischemic stroke accounts for 20–30% of all strokes, resulting in a serious economic burden on both families and society.1–3 Studies have shown that more than 90% of thrombi in NVAF patients originate from the left atrium,4 and that in preventing AF-related stroke, the therapeutic effects of left atrial appendage closure (LAAC) are non-inferior to that of classical treatment with warfarin.5–7 Therefore, LAAC is an effective means of preventing stroke. For patients with AF who are at risk of stroke and cannot take long-term anticoagulant therapy, LAAC is an ideal supplement or alternative and has become a popular stroke prevention treatment for patients with AF worldwide.8,9

Currently, there are no definitive guidelines or recommendations regarding anticoagulation therapy following LAAC. The commonly used clinical anticoagulation regimens all refer to the PROTECT-AF study.10,11 This study suggests the continuation of warfarin anticoagulation therapy for 45 days following surgery and discontinuation of clopidogrel after six months of dual antiplatelet therapy (DAPT), followed by replacement of DAPT with lifelong oral aspirin. Patients who are not suitable for warfarin anticoagulation can have DAPT with aspirin and clopidogrel. After six months of therapy, clopidogrel can be discontinued and replaced with lifelong oral aspirin as single drug therapy. However, there is no definitive clinical evidence for the use of the novel non-vitamin K antagonist oral anticoagulants (NOACs) following LAAC.

This study was a single-center, retrospective cohort study designed to preliminarily investigate the safety and efficacy of the oral NOACs following LAAC.

Subjects and Methods

Study Subjects

A total of 74 patients who underwent LAAC in the arrhythmia subspecialty at the First Affiliated Hospital of the University of Science and Technology of China between November 2018 and September 2019 were enrolled in the study. In four patients, implantations failed due to a large orifice or the shallow depth of the left atrial appendage (LAA). The remaining 70 patients with NVAF underwent successful surgery with implantation of a Watchman occluder (Boston Scientific, US), and all patients completed a 45-day postoperative outpatient follow-up period. Patients were grouped according to the postoperative anticoagulation regimen they received: there were 40 patients in the NOACs group and 30 patients in the warfarin group. Sixteen for dabigatran and 24 for rivaroxaban (27–3 (not successfully implanted)). As the anticoagulant mechanism of the new oral anticoagulants differs from that of warfarin, the international normalized ratio (INR) is not used as the evaluation standard. For NOAC therapy either dabigatran or rivaroxaban were used: dabigatran 110 mg bid, rivaroxaban 20 mg bid (or 15 mg bid if CRCI < 30–49mL/min).

The study was conducted in accordance with the Declaration of Helsinki (revised 2013) and was approved by the Ethics Committee of the First Affiliated Hospital of the University of Science and Technology of China. All patients gave informed consent.

Inclusion Criteria Were as Follows

(1) age ≥18 years, (2) a confirmed diagnosis of NVAF within the previous six months, (3) a HAS-BLED score of ≥3, (4) a CHA2DS2-VAS score of ≥2, (5) a contraindication to, unsuitability for, or poor compliance with NOACs, and (6) a contraindication to warfarin or the inability to take long term warfarin.

Following LAAC, all patients should receive oral anticoagulation with warfarin or NOACs as recommended and be followed up in outpatient clinic 45 days post-surgery.

Exclusion Criteria Were as Follows

(1) valvular AF, (2) AF caused by a reversible etiology, (3) thrombosis in the left atrium, (4) new onset ischemic stroke within the previous month, (5) other conditions necessitating the long-term use of anticoagulants, (6) life expectancy <1 year, (7) unobstructed foramen ovale or septal defect, and (8) left ventricular ejection fraction (LVEF) <35% or patients in NYHA functional class Ⅳ and temporarily uncorrected.12–14

Methods

Collection of Data

A retrospective survey method was used to retrieve cases from the hospital information system that met the above criteria. The general characteristics of each patient were collected, including hospitalization ID, name, gender, age, surgery date, AF type, history of AF ablation, medical history (hypertension, diabetes, congestive heart failure, stroke, TIA, thromboembolism, hepatic and renal dysfunction, ischemic stroke, hemorrhage or hemorrhagic tendency), left atrium internal diameter, LVEF, CHA2DS2-VASc score, and HAS-BLED score. Microsoft Excel software was used to categorize and integrate the collected data and postoperative follow-up data with the composition ratios being analyzed.

LAAC

All patients gave informed written consent for the surgery. Trans-esophageal echocardiography (TEE) was routinely performed to examine the morphology and structure of the LAA in order to exclude contraindications.15 The surgery was performed under general or local anesthesia and involved femoral vein puncture. The long sheath was implanted, and the guidewire was delivered through a septal puncture under TEE guidance. The long sheath was then replaced with a 14F guiding sheath. The diameter of the LAA, the depth of the LAA, and the size of the anchoring area were measured by TEE or intra-cardiac echocardiography. The appropriate Watchman LAA occluder was selected, implanted under real-time ultrasonography guidance, and released once it was in place. The pull test was conducted to verify firm anchoring of the occluder. Finally, the existence of residual blood flow around the occluder and the presence of a pericardial effusion was identified. If the condition was in accordance with the PASS principle, the occluder was released, and the patient was transferred to CCU.

The Intraoperative and Postoperative Anticoagulation Therapy: All Patients Received a Continuous Anticoagulation Regimen

During the operation, heparin was used for anticoagulation: a loading dose of 100 U/kg was given followed by an infusion of 1000 U/h. The activated coagulation time was monitored dynamically and maintained within the range of 250~300 s. Six hours after the operation, low molecular weight heparin was given based on body weight. For those without contraindications for warfarin, low molecular weight heparin was discontinued when the INR reached 1.5 and warfarin was continued with the INR maintained at 2.0~3.0. For those who could not take warfarin orally, dabigatran 110 mg bid or rivaroxaban 15 mg once daily was given as a single replacement drug. All patients were followed up 45 days after the operation. In cases where no residual shunt or thrombosis on the surface of the occluder was identified, the warfarin or NOACs were discontinued and the patient was started on DAPT (aspirin 100 mg QD + clopidogrel 75 mg QD) up until six months post-surgery. Aspirin was then given as oral monotherapy.

The 45-Day Postoperative Follow-Up

Routine follow-up visits were conducted in the outpatient clinic 45 days after LAAC. The clinical medical history was reviewed, including whether there had been any adverse events such as ischemic stroke, various hemorrhages, or device-related thrombosis (DRT). Color Doppler echocardiography and an electrocardiogram were performed and coagulation function was tested.

Observation Indicators

During the operation, the occlusion rate, the compression ratio of the occluder, the fall-off of the device, the residual shunt volume (if present), the volume of pericardial effusion (if present), the formation of DRT, along with other complications including stroke and vascular injury were observed. At 45 days following surgery, the formation of DRT, residual shunts and the volume of the shunts, stroke and its complications (including digestive, intracranial, nasal, or gum hemorrhage), subcutaneous hematoma, and mucosal ecchymosis were noted.

Statistical Analysis

SPSS 18.0 software was used for data analysis. The measurement variables conforming to the normal distribution were expressed by (x ± s), and independent sample t-tests were used for comparison between groups. The continuous variables that did not conform to the normal distribution were represented by the median (the minimum, the maximum), and the rank-sum test was used for comparison between groups. P < 0.05 was considered statistically significant.

Results

Comparison of the Baseline Characteristics Between the Two Groups (Table 1)

There was no statistical difference in age, gender, history of hypertension, stroke, TIA, thromboembolism, vascular disease, congestive heart failure, diabetes mellitus, hepatic and renal dysfunction, stroke and hemorrhage, unstable INR, CHA2DS2-VASc scores, HAS-BLED scores, the inner diameter of the left atrium, LVEF, the diameter of the LAA orifice, and the depth of the LAA between the two groups (P > 0.05 in all).

Comparison of the Intraoperative Data Between the Two Groups (Table 2)

There was no statistically significant difference in the occlusion rate, the compression ratio of the occluder, the incidence of residual shunts and DRT, the incidence of stroke and pericardial effusion, and the volume of effusion between the two groups (P > 0.05 in all). The volume of residual shunts in the NOACs group was significantly lower than that in the warfarin group (P = 0.04).

Comparison of the Data During the 45-Day Postoperative Follow-Up Between the Two Groups (Table 3)

There was no significant difference in the incidence of DRT, the volume of residual shunts, ischemic stroke incidence, and hemorrhagic stroke between the two groups (P > 0.05 in all). The volume of the residual shunt in the NOACs group was significantly lower than that in the warfarin group (P = 0.034). The incidence of minor hemorrhage and the incidence of total hemorrhage in the NOACs group were significantly lower than those in the warfarin group (5% vs 30%, P = 0.004, and 5% vs 33.3%, P = 0.002, respectively).

Discussion

Currently, the prevalence of AF in China is approximately 0.77%.15,16 The prevalence and incidence of AF increases progressively with age. Strokes and thromboembolic events are both common and serious complications of AF. Anticoagulation therapy can significantly reduce the incidence of both stroke and systemic embolic events, thereby improving prognosis. LAAC is non-inferior to classical warfarin therapy in preventing AF-related stroke. The 2018 Chinese guidelines for the diagnosis and treatment of AF3 also recommend LAAC as an intervention for patients with NVAF who have contraindications to long-term anticoagulation therapy and are at high risk of stroke. Anticoagulation therapy is necessary following LAAC to prevent DRT. A multicenter review by Fauchier et al17 revealed a 7.2% incidence of DRT following occlusion. The PROTECT-AF study suggested that the incidence of DRT was 4.2% (2.4%~6.8%).18,19 In this study, one case of DRT was identified in the warfarin group from the intraoperative data. During the 45-day postoperative follow-up period, the incidence of DRT was 4.3%, with one case in the NOACs group and two cases in the warfarin group. There was no statistical difference between the two groups. There was a correlation between the formation of DRT following occlusion and patient factors, inadequate postoperative anticoagulation therapy, and the device adopted.

Currently, there are differences in global anticoagulation regimens following LAAC, although it is agreed that patients should continue to take warfarin for anticoagulation for 45 days after the procedure.20 However, there are many problems associated with the oral administration of warfarin including its narrow therapeutic window and the fact that food and medication can affect its efficacy and safety. Consequently, monitoring the coagulation effect of warfarin using the INR and implementing an individualized drug regimen is necessary.21 Moreover, the risk of hemorrhage is high with warfarin therapy, and this increases with age. In recent years, a series of NOACs have emerged providing a new option for clinical anticoagulation therapy.22 Clinical studies have verified that NOACs are equally effective and safer than warfarin in preventing thromboembolism in patients with AF.23,24

However, there is no clear evidence on the safety and efficacy of NOACs in preventing DRT following LAAC. Related studies have shown that the incidence of thromboembolic events is reduced. There is no difference in hemorrhagic events when NOACs are used following LAAC as compared with the traditional postoperative anticoagulation regimen of warfarin.10 A multicenter, observational study conducted by Fountain et al showed no thromboembolic events, such as cerebral infarction or atrial thrombosis, in the group implanted with the Watchman occluder as compared with the group implanted with the ACP occluder. Compared to the warfarin group, the dabigatran group had a lower incidence of hemorrhagic events (18.2% vs 2.9%, respectively, P = 0.04), while the rivaroxaban group showed a tendency towards a lower incidence of hemorrhagic events (0%, P = 0.08). A subgroup analysis of the EWOLUTION study suggested no correlation between the occurrence of postoperative hemorrhagic events after LAAC and the application of anticoagulation protocols. However, the lowest incidence of postoperative hemorrhagic events occurred in the oral NOACs group. A small number of foreign studies have compared different anticoagulation protocols following LAAC and found no statistically significant differences in the incidences of DRT formation or embolic events between the different anticoagulation protocols. When investigating patients who underwent a 45-day postoperative follow-up with LAAC, Bösche et al25 found that no stroke events occurred in patients treated with early NOACs therapy and those treated with aspirin and clopidogrel. A retrospective study by Enomoto et al26 found no statistically significant differences in the incidences of device surface thrombosis, thromboembolic events, or hemorrhagic events between patients anti-coagulated with NOACs and those treated with oral warfarin anticoagulation following LAAC. The efficacy and safety of NOACs for percutaneous LAAC remains poorly reported in China. In this study, when the intraoperative data were compared between the two groups, one case of stroke was identified in the NOACs group and none in the warfarin group. At 45-day follow-up, one case of ischemic stroke was identified in the NOACs group and none in the warfarin group. Results also showed that the residual shunt volume was significantly reduced in the NOACs group as compared with the warfarin group, which might contribute to the reduced risk of embolization and hemorrhage. Compared with the data in the warfarin group, the incidences of minor small hemorrhagic events and total hemorrhagic events were significantly reduced in the NOACs group, and the incidence of adverse events was significantly lower. The results during the follow-up period were generally consistent with the above studies.

In this study, limitations included the small sample size and the short duration of follow-up. Larger sample sizes and long-term follow-up data are needed in the future to support the validity and safety of NOACs following LAAC.

Conclusion

In this study, the use of NOACs for postoperative anticoagulation therapy following LAAC did not increase the risk of embolization or hemorrhage during the short-term follow-up period.

Table 1

Comparison of Baseline Data Between the Two Groups

Items	The NOACs Group (40 Cases)	The Warfarin Group (30 Cases)	p value
Age [Year, M (The maximum, the minimum)]	67(80,50)	65(86,46)	0.38
Male [Case (%)]	21(52.5)	20(66.7)	0.20
Hypertension [Case (%)]	24(60)	24(80)	0.85
Diabetes mellitus [Case (%)]	10(25)	4(13.3)	0.23
History of stroke [Case (%)]	31(77.5)	21(70)	0.48
Congestive heart failure [Case (%)]	3(7.5)	1(3.3)	0.46
History of vascular diseases	5(12.5)	6(20)	0.40
Stroke/TIA/Thromboembolism[Case (%)]	31(77.5)	22(73.3)	0.69
History of hemorrhage [Case (%)]	5(12.5)	4(13.3)	0.92
History of unstable INR	10(25)	4(13.3)	0.23
Combination of aspirin or non-steroidal anti-inflammatory drugs	21(52.5)	17(56.7)	0.73
Alcoholism	1(2.5)	1(3.3)	0.84
CHA2DS2-VASc scores	4.05±1.09	3±1.37	0.86
HAS-BLED scores	3.18±0.59	3.17±0.82	0.96
The inner diameter of the left atrium	47.23±5.66	49.07±7.43	0.25
LVEF	64.45±9.43	62.4±8.24	0.35
The diameter of the left atrial appendage orifice	25.28±4.27	23.93±3.68	0.18
Length of the left atrial appendage	26.63±2.60	25.13±3.75	0.06
Atrial fibrillation [Case (%)]	30 (75)	19 (63.3)	0.42
Chronic renal dysfunction (creatinine clearance rate <80mL/min) [Case (%)]	3 (7.5)	5 (16.7)	0.40
Coronary heart disease	8 (20)	7 (23.3)	0.36

Table 2

Intraoperative Data of the Two Groups Were Compared

Items	The NOACs Group (40 Cases)	The Warfarin Group (30 Cases)	p value
The compression ratio of the occluder (%, x±s)	16.20±4.78	15.56±4.38	0.71
The occlusion rate [Case (%)]	40(100)	29(96.7)	>0.99
The residual shunts [Case (%)]	5(12.5)	8(26.7)	0.12
The volume of the residual shunts [mm, M (the maximum, the minimum)]	0(0,3)	0(0,4.5)	0.04
Device-related thrombosis [Case (%)]	0(0)	1(3.3)	0.25
Stroke [Case (%)]	1(2.5)	0(0)	0.39
Pericardial effusion [Case (%)]	1(2.5)	2(6.6)	0.40
The volume of the pericardial effusion [mm, M (the maximum, the minimum)]	0(0,5)	0(0,11)	0.31

Abbreviation: NOACs, novel oral anticoagulants.

Table 3

Comparison of Follow-Up Data 45 Days After Operation Between the Two Groups

Items	The NOACs Group (40 Cases)	The Warfarin Group (30 Cases)	p value
The residual shunts [Case (%)]	3(7.5)	7(23.3)	0.06
The volume of the residual shunts [mm, M (the maximum, the minimum)]	0(0,2)	0(0,3)	0.03
Device-related thrombosis [Case (%)]	1(2.5)	2(6.7)	0.4
Ischemic stroke [Case (%)]	1(2.5)	0(0)	0.39
Cerebral hemorrhage [Case (%)]	0(0)	1(3.3)	0.25
Minor hemorrhage [Case (%)]	2(5)	9(30)	0.004
Total hemorrhage [Case (%)]	2(5)	10(33.3)	0.002