Literature DB >> 33725790

Efficient transformation of Lactobacillus sake by electroporation.

Françoise Berthier1, Monique Zagorec1, Marie Champomier-Vergès1, S D Ehrlich2, Françoise Morel-Deville1.   

Abstract

A procedure to transform intact Lactobacillus sake cells by electroporation was developed through a systematic examination of the effect of changes in various parameters on the transformation efficiency of Lact. sake strain 64F. The most critical factors were found to be the electrical parameters, the composition of washing and electroporation/storage solutions, and the presence of MgCI2 in the expression medium. Under optimal conditions transformation efficiencies up to 107 transformants (μg supercoiled DNA)-1 were obtained. The optimized procedure was successfully applied to other Lact. sake strains and consistently yielded from 104 to 107 transformants (μg supercoiled DNA)-1.

Entities:  

Year:  1996        PMID： 33725790     DOI： 10.1099/13500872-142-5-1273

Source DB:  PubMed          Journal:  Microbiology (Reading)        ISSN： 1350-0872            Impact factor:   2.777


  4 in total

1.  A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria.

Authors:  Andrés Miguel-Arribas; Jorge Val-Calvo; César Gago-Córdoba; José M Izquierdo; David Abia; Ling Juan Wu; Jeff Errington; Wilfried J J Meijer
Journal:  Nucleic Acids Res       Date:  2021-06-04       Impact factor: 16.971

Review 2.  Recent progress on n-butanol production by lactic acid bacteria.

Authors:  Qi Li; Jieze Zhang; Junjie Yang; Yu Jiang; Sheng Yang
Journal:  World J Microbiol Biotechnol       Date:  2021-10-26       Impact factor: 3.312

3.  A single change in the aptamer of the Lactiplantibacillus plantarum rib operon riboswitch severely impairs its regulatory activity and leads to a vitamin B2 - overproducing phenotype.

Authors:  Inés Ripa; José Ángel Ruiz-Masó; Nicola De Simone; Pasquale Russo; Giuseppe Spano; Gloria Del Solar
Journal:  Microb Biotechnol       Date:  2021-10-02       Impact factor: 5.813

4.  Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers.

Authors:  Spase Stojanov; Tina Vida Plavec; Julijana Kristl; Špela Zupančič; Aleš Berlec
Journal:  Int J Mol Sci       Date:  2021-12-20       Impact factor: 5.923
  4 in total

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