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Literature DB >> 33723275

Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells.

Heping Cao¹, Kandan Sethumadhavan², Fangping Cao³, Thomas T Y Wang⁴.

Abstract

Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene expression in cancer cells. In this study, human colon cancer cells (COLO 225) were treated with gossypol. MTT assay showed significant inhibitory effect under high concentration and longtime treatment. We analyzed the expression of 55 genes at the mRNA level in the cells; many of them are regulated by gossypol or ZFP36/TTP in cancer cells. BCL2 mRNA was the most stable among the 55 mRNAs analyzed in human colon cancer cells. GAPDH and RPL32 mRNAs were not good qPCR references for the colon cancer cells. Gossypol decreased the mRNA levels of DGAT, GLUT, TTP, IL families and a number of previously reported genes. In particular, gossypol suppressed the expression of genes coding for CLAUDIN1, ELK1, FAS, GAPDH, IL2, IL8 and ZFAND5 mRNAs, but enhanced the expression of the gene coding for GLUT3 mRNA. The results showed that gossypol inhibited cell survival with decreased expression of a number of genes in the colon cancer cells.

Entities: Chemical

Mesh：

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Year: 2021 PMID： 33723275 PMCID： PMC7961146 DOI： 10.1038/s41598-021-84970-8

Source DB: PubMed Journal: Sci Rep ISSN： 2045-2322 Impact factor: 4.996

99 in total

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Journal: Plant Foods Hum Nutr Date: 1996-01 Impact factor: 3.921

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Journal: Poult Sci Date: 2014-06-05 Impact factor: 3.352

Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells.

Review 1. Cottonseed protein: what does the future hold?

2. RNAi-mediated Ultra-low gossypol cottonseed trait: performance of transgenic lines under field conditions.

Review 3. Gossypol: a contraceptive for men.

4. [Utilization of gossypol-free cottonseed and its by-products as human food].

Review 5. Effects of gossypol and cottonseed products on reproduction of mammals.

6. Minimum nitrogen requirement from glandless cottonseed protein for nitrogen balance in college women.

7. Hepatocarcinogenicity of glandless cottonseeds and cottonseed oil to rainbow trout (Salmo gairdnerii).

8. Effects of a glandless cottonseed protein diet on fasting plasma amino acid levels in college women.

9. Effects of dietary gossypol concentration on growth performance, blood profiles, and hepatic histopathology in meat ducks.

10. Toxicity of Gossypol from Cottonseed Cake to Sheep Ovarian Follicles.

1. Identification of bicyclic compounds that act as dual inhibitors of Bcl-2 and Mcl-1.

2. Gossypol Acetic Acid Attenuates Cardiac Ischemia/Reperfusion Injury in Rats via an Antiferroptotic Mechanism.

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Review 4. Role of Plant-Derived Active Constituents in Cancer Treatment and Their Mechanisms of Action.

5. ZFAND5 Is an Independent Prognostic Biomarker of Perihilar Cholangiocarcinoma.

6. Cottonseed extracts regulate gene expression in human colon cancer cells.