Guanghong Du1, Xuelian Yu1, Yun Chen1, Wangting Cai2. 1. Department of Geriatrics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. 2. Organ transplant center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Abstract
BACKGROUND: Colorectal cancer (CRC) is regarded as one of the most common malignancies in the world. MiR-1-3p was reported to be a tumor suppressor in CRC. However, the mechanisms have not been fully elucidated. METHODS: To identify CRC-associated miRNA, microarray data set GSE30454 was downloaded from the Gene Expression Omnibus database (GEO), and miR-1-3p was screened out as a candidate. The expression of miR-1-3p was detected using quantitative real-time polymerase chain reaction (qRT-PCR) in CRC cell lines and tissues. CCK-8 assay and transwell invasion assay were performed to determine CRC cell line proliferation and invasion, respectively. The levels of YWHAZ and EMT-associated proteins were detected using western blotting. RESULTS: Bioinformatic analysis showed that miR-1-3p was downregulated in CRC tissues, which is verified by our experimental validation. The overexpression of miR-1-3p significantly suppressed CRC cell proliferation and invasion. Further studies showed that YWHAZ was a direct target of miR-1-3p and mediated epithelial-mesenchymal transition (EMT) modulated by miR-1-3p. CONCLUSION: Our results demonstrated that miR-1-3p suppresses colorectal cancer cell proliferation and metastasis through regulating YWHAZ-mediated EMT, which may support a novel therapeutic strategy for CRC patients.
BACKGROUND: Colorectal cancer (CRC) is regarded as one of the most common malignancies in the world. MiR-1-3p was reported to be a tumor suppressor in CRC. However, the mechanisms have not been fully elucidated. METHODS: To identify CRC-associated miRNA, microarray data set GSE30454 was downloaded from the Gene Expression Omnibus database (GEO), and miR-1-3p was screened out as a candidate. The expression of miR-1-3p was detected using quantitative real-time polymerase chain reaction (qRT-PCR) in CRC cell lines and tissues. CCK-8 assay and transwell invasion assay were performed to determine CRC cell line proliferation and invasion, respectively. The levels of YWHAZ and EMT-associated proteins were detected using western blotting. RESULTS: Bioinformatic analysis showed that miR-1-3p was downregulated in CRC tissues, which is verified by our experimental validation. The overexpression of miR-1-3p significantly suppressed CRC cell proliferation and invasion. Further studies showed that YWHAZ was a direct target of miR-1-3p and mediated epithelial-mesenchymal transition (EMT) modulated by miR-1-3p. CONCLUSION: Our results demonstrated that miR-1-3p suppresses colorectal cancer cell proliferation and metastasis through regulating YWHAZ-mediated EMT, which may support a novel therapeutic strategy for CRC patients.
Authors: Yong Zhao; Joshua F Ransom; Ankang Li; Vasanth Vedantham; Morgan von Drehle; Alecia N Muth; Takatoshi Tsuchihashi; Michael T McManus; Robert J Schwartz; Deepak Srivastava Journal: Cell Date: 2007-03-29 Impact factor: 41.582
Authors: Aaron L Sarver; Amy J French; Pedro M Borralho; Venugopal Thayanithy; Ann L Oberg; Kevin A T Silverstein; Bruce W Morlan; Shaun M Riska; Lisa A Boardman; Julie M Cunningham; Subbaya Subramanian; Liang Wang; Tom C Smyrk; Cecilia M P Rodrigues; Stephen N Thibodeau; Clifford J Steer Journal: BMC Cancer Date: 2009-11-18 Impact factor: 4.430