Literature DB >> 3371661

Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker.

S de la Luna1, I Soria, D Pulido, J Ortín, A Jiménez.   

Abstract

Recombinant plasmids have been obtained that lead to the accumulation of five- to ten-fold more puromycin-N-acetyl-transferase (PAC) mRNA and two- to three-fold more PAC activity than the already described plasmid pSV2pac [Vara et al., Nucl. Acids Res. 14 (1986) 4117-4124]. When these optimized recombinants were used for stable transformation to puromycin resistance, efficiencies up to 1 x 10(-2) were obtained, indicating that these pac-containing recombinants may be very useful dominant selectable markers for gene transfer in mammalian cells.

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Year:  1988        PMID： 3371661     DOI： 10.1016/0378-1119(88)90585-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  45 in total

1.  Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells.

Authors:  I Frolov; E Agapov; T A Hoffman; B M Prágai; M Lippa; S Schlesinger; C M Rice
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

2.  Functional replacement of nucleocapsid flanking regions by heterologous counterparts with divergent primary sequences: effects of chimeric nucleocapsid on the retroviral replication cycle.

Authors:  William Fu; Wei-Shau Hu
Journal:  J Virol       Date:  2003-01       Impact factor: 5.103

3.  Complementarity-directed RNA dimer-linkage promotes retroviral recombination in vivo.

Authors:  Jacob Giehm Mikkelsen; Søren Vestergaard Rasmussen; Finn Skou Pedersen
Journal:  Nucleic Acids Res       Date:  2004-01-09       Impact factor: 16.971

4.  High-frequency intracellular transposition of a defective mammalian provirus detected by an in situ colorimetric assay.

Authors:  T Tchenio; T Heidmann
Journal:  J Virol       Date:  1992-03       Impact factor: 5.103

5.  Use of puromycin N-acetyltransferase (PAC) as a new reporter gene in transgenic animals.

Authors:  E Gómez Lahoz; M S López de Haro; A Nieto; P Esponda
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

6.  The insulin-like growth factor I receptor as a physiologically relevant target of p53 in apoptosis caused by interleukin-3 withdrawal.

Authors:  M Prisco; A Hongo; M G Rizzo; A Sacchi; R Baserga
Journal:  Mol Cell Biol       Date:  1997-03       Impact factor: 4.272

7.  Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function.

Authors:  F Liu; R A Roth
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

8.  Modified lentiviral LTRs allow Flp recombinase-mediated cassette exchange and in vivo tracing of "factor-free" induced pluripotent stem cells.

Authors:  Johannes Kuehle; Soeren Turan; Tobias Cantz; Dirk Hoffmann; Julia D Suerth; Tobias Maetzig; Daniela Zychlinski; Christoph Klein; Doris Steinemann; Christopher Baum; Juergen Bode; Axel Schambach
Journal:  Mol Ther       Date:  2014-01-17       Impact factor: 11.454

9.  Association of insulin receptor substrate 1 with simian virus 40 large T antigen.

Authors:  Z L Fei; C D'Ambrosio; S Li; E Surmacz; R Baserga
Journal:  Mol Cell Biol       Date:  1995-08       Impact factor: 4.272

10.  Gene transfer into mammalian cells by a Rous sarcoma virus-based retroviral vector with the host range of the amphotropic murine leukemia virus.

Authors:  E V Barsov; S H Hughes
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103
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