| Literature DB >> 33713513 |
Zhaoyang Wu1, Xinzhu Xu1, Peng Xi1,2.
Abstract
Stimulated emission depletion (STED) microscopy allows high lateral and axial resolution, long term imaging in living cells. Here we review recent technical advances in STED microscopy, with emphasis on resolution and measurement range of XYZt four dimensions. Different STED technical advances and novel STED probes are discussed with their respective application in biological subcellular imaging. This review may serve as a practical guide for choosing a suitable approach to the advanced STED super-resolution imaging.Keywords: STED; axial resolution; lateral resolution; measurement range; temporal resolution
Year: 2021 PMID： 33713513 DOI： 10.1002/jemt.23750
Source DB: PubMed Journal: Microsc Res Tech ISSN： 1059-910X Impact factor: 2.769