Sara Calhoun1,2, Tisza Ann Szeremy Bell3,4, Lukas R Dahlin5, Yuliya Kunde3, Kurt LaButti1, Katherine B Louie1, Andrea Kuftin1, Daniel Treen1, David Dilworth1, Sirma Mihaltcheva1, Christopher Daum1, Benjamin P Bowen1, Trent R Northen1, Michael T Guarnieri5, Shawn R Starkenburg6, Igor V Grigoriev7,8,9. 1. US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. 2. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. 3. Applied Genomics Team, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA. 4. Division of Biological Sciences, Genome Core, University of Montana, Missoula, MT, USA. 5. National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, USA. 6. Applied Genomics Team, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA. shawns@lanl.gov. 7. US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. ivgrigoriev@lbl.gov. 8. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. ivgrigoriev@lbl.gov. 9. Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA, USA. ivgrigoriev@lbl.gov.
Abstract
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.
Microalgae efficiently convert sunlight into n>an class="Chemical">lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.
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