Fahimeh Pourjafari1, Tahereh Haghpanah1, Fariba Sharififar2, Seyed Noreddin Nematollahi-Mahani1, Ali Afgar3, Massood Ezzatabadipour4. 1. Anatomical Sciences Department, School of Medicine, Kerman University of Medical Sciences, P.O. Box:76169-14115, Kerman, Iran. 2. Herbal and Traditional Medicines Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran. 3. Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. 4. Anatomical Sciences Department, School of Medicine, Kerman University of Medical Sciences, P.O. Box:76169-14115, Kerman, Iran. ezzatabadipm@gmail.com.
Abstract
BACKGROUND: The aim of the present study was to assess the expression and serum level of AMH in first-generation female mice pups following fennel and flaxseed consumption. METHODS: Twenty pregnant NMRI mice were allocated into four groups including control (CTL), fennel (FV), flaxseed (LU) and FV+ LU. Sixty-four female offsprings after lactation period, received the same regimen as their mothers for 56 and 240 days. The ovarian follicles development, serum concentration of AMH, as well as gene and protein expression of AMH were evaluated in the female offsprings at post-natal day 56 (PND56) and 240 (PND240). RESULTS: The number of total growing follicles were raised in the FV group in compression to the all experimental groups. In contrast, LU group showed a marked decrease in their numbers. The highest level of serum AMH was seen in the FV-diet mice, whereas LU negatively affected it. The expression level of AMH also increased in the FV and FV + LU groups, while a reduction was observed in the LU group. As well, IHC data showed that the number of AMH-positive cells in almost ovarian follicles of FV and FV + LU-treated mice was in compared to those of the LU group. CONCLUSIONS: The overall effect of fennel treatment (alone and in combination with flaxseed) on ovary might be maintain primordial follicle storage through increased expression and serum level of AMH.
BACKGROUND: The aim of the present study was to assess the expression and serum level of AMH in first-generation female mice pups following fennel and flaxseed consumption. METHODS: Twenty pregnant NMRI mice were allocated into four groups including control (CTL), fennel (FV), flaxseed (LU) and FV+ LU. Sixty-four female offsprings after lactation period, received the same regimen as their mothers for 56 and 240 days. The ovarian follicles development, serum concentration of AMH, as well as gene and protein expression of AMH were evaluated in the female offsprings at post-natal day 56 (PND56) and 240 (PND240). RESULTS: The number of total growing follicles were raised in the FV group in compression to the all experimental groups. In contrast, LU group showed a marked decrease in their numbers. The highest level of serum AMH was seen in the FV-diet mice, whereas LU negatively affected it. The expression level of AMH also increased in the FV and FV + LU groups, while a reduction was observed in the LU group. As well, IHC data showed that the number of AMH-positive cells in almost ovarian follicles of FV and FV + LU-treated mice was in compared to those of the LU group. CONCLUSIONS: The overall effect of fennel treatment (alone and in combination with flaxseed) on ovary might be maintain primordial follicle storage through increased expression and serum level of AMH.
Authors: Janduí Escarião da Nóbrega; Paulo Bayard Dias Gonçalves; Roberta Nogueira Chaves; Deborah de Melo Magalhães; Rafael Rossetto; Isabel Bezerra Lima-Verde; Gabriel Ribas Pereira; Cláudio Cabral Campello; José Ricardo Figueiredo; João Francisco Coelho de Oliveira Journal: Zygote Date: 2011-03-18 Impact factor: 1.442
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