Esther Sook Kuan Kok1, Xian Jin Lim1, Soo Xiong Chew1, Shu Fen Ong1, Lok Yin See1, Siao Hua Lim1, Ling Ang Wong1, Fabian Davamani2, Venkateshbabu Nagendrababu1,3, Amr Fawzy4, Umer Daood5. 1. Division of Clinical Dentistry, Schoolof Dentistry, International Medical University Kuala Lumpur, Kuala Lumpur, Malaysia. 2. Faculty of Biomedical Science, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia. 3. Department of Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah, Sharjah, UAE. 4. UWA Dental School, University of Western Australia, Nedlands, Australia. 5. Division of Clinical Dentistry, Schoolof Dentistry, International Medical University Kuala Lumpur, Kuala Lumpur, Malaysia. umerdaood@imu.edu.my.
Abstract
BACKGROUND: Compare antimicrobial efficacy of a quarternary ammonium silane (QAS)/k21 as an intracanal medicament against E. faecalis and C. albicans biofilms formed on root dentin. METHODOLOGY: Dentin blocks were sterilized and E. faecalis and C. albicans microbial colonies were counted for colony-forming-units against 2%k21, 2%CHX and Ca(OH)2 medicaments. Biofilm colonies after 7 days on dentin were analysed using confocal laser scanning microscopy with live/dead bacterial viability staining. TEM was done to study dentin collagen matrix. Dentin discs from 3rd day and 7th day well plate was used for Raman spectra and observed under fluorescent-microscope. Docking studies were carried out on MMP-2 S1 binding-domain with k21. RESULTS: There was reduction of E. faecalis/C. albicans when k21, chlorhexidine and calcium hydroxide were used with highest percentage in 2%k21 treated specimens. 2%k21 showed dense and regular collagen network with intact cross-banding and decreased Raman intensity for 2%k21 on 3rd day. NaOCl + k21 showed least adherence, whereas saline groups showed highest adherence of E. faecalis and C. albicans to root-canal dentin. Alizarin red staining of hDPSCs revealed calcium deposition in all groups with significant difference seen amongst 2%k21 groups. MMP-2 ligand binding was seen accurately indicating possible target sites for k21 intervention. CONCLUSION: 2%k21 can be considered as alternative intracanal medicament.
BACKGROUND: Compare antimicrobial efficacy of a quarternary ammonium silane (QAS)/k21 as an intracanal medicament against E. faecalis and C. albicans biofilms formed on root dentin. METHODOLOGY: Dentin blocks were sterilized and E. faecalis and C. albicans microbial colonies were counted for colony-forming-units against 2%k21, 2%CHX and Ca(OH)2 medicaments. Biofilm colonies after 7 days on dentin were analysed using confocal laser scanning microscopy with live/dead bacterial viability staining. TEM was done to study dentin collagen matrix. Dentin discs from 3rd day and 7th day well plate was used for Raman spectra and observed under fluorescent-microscope. Docking studies were carried out on MMP-2 S1 binding-domain with k21. RESULTS: There was reduction of E. faecalis/C. albicans when k21, chlorhexidine and calcium hydroxide were used with highest percentage in 2%k21 treated specimens. 2%k21 showed dense and regular collagen network with intact cross-banding and decreased Raman intensity for 2%k21 on 3rd day. NaOCl + k21 showed least adherence, whereas saline groups showed highest adherence of E. faecalis and C. albicans to root-canal dentin. Alizarin red staining of hDPSCs revealed calcium deposition in all groups with significant difference seen amongst 2%k21 groups. MMP-2 ligand binding was seen accurately indicating possible target sites for k21 intervention. CONCLUSION: 2%k21 can be considered as alternative intracanal medicament.
Authors: N Vivacqua-Gomes; C C R Ferraz; B P F A Gomes; A A Zaia; F B Teixeira; F J Souza-Filho Journal: Int Endod J Date: 2002-09 Impact factor: 5.264
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Authors: Umer Daood; Abhishek Parolia; Jukka Matinlinna; Cynthia Yiu; Hany Mohamed Aly Ahmed; Amr Fawzy Journal: Dent Mater Date: 2020-10-01 Impact factor: 5.304
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