Xiaoqing Li1, Yinghui Kong2, He Li2, Manyuan Xu2, Ming Jiang2, Weiguo Sun2, Suping Xu2. 1. Department of Dermatology, The Affiliated Huaian NO.1 People's Hospital of Nanjing Medical University, No. 6, West Beijing Road, Huaiyin District, Huaian, 223300, Jiangsu, China. xiaonuomon@163.com. 2. Department of Dermatology, The Affiliated Huaian NO.1 People's Hospital of Nanjing Medical University, No. 6, West Beijing Road, Huaiyin District, Huaian, 223300, Jiangsu, China.
Abstract
BACKGROUND: Cutaneous squamous cell carcinoma (CSCC) is a severe malignancy derived from skin. Dysregulated circular RNAs (circRNAs) might play vital roles in tumor development. OBJECTIVE: Here, we aimed to explore the function of a novel circRNA circ_0067772 in CSCC. METHODS: Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to determine the expression of circ_0067772, microRNA (miR)-1238-3p and forkhead box protein G1 (FOXG1). Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Transwell assay and wound healing assay were employed to examine cell metastasis. Flow cytometry was employed to monitor cell cycle and apoptosis. The target association between miR-1238-3p and circ_0067772 or FOXG1 was validated by dual-luciferase reporter assay. Moreover, role of circ_0067772 in vivo was investigated via xenograft model in nude mice. RESULTS: Circ_0067772 and FOXG1 were upregulated, while miR-1238-3p was downregulated in CSCC tissues and cells. Circ_0067772 knockdown conferred inhibitory effects on cell proliferation, migration and invasion of CSCC cells. MiR-1238-3p served as a target of circ_0067772, whose silencing could reverse circ_0067772 knockdown-induced inhibitory impact on the malignant cellular behaviors. Circ_0067772 positively regulated FOXG1 expression by antagonizing miR-1238-3p. Additionally, miR-1238-3p could repress CSCC cell proliferation, migration and invasion by targeting FOXG1. Also, circ_0067772 knockdown hindered CSCC tumor growth in vivo. CONCLUSION: Our study identified a novel oncogenic circRNA and the involvement of circ_0067772/miR-1238-3p/FOXG1 axis in CSCC development, providing a target for CSCC therapy.
BACKGROUND: Cutaneous squamous cell carcinoma (CSCC) is a severe malignancy derived from skin. Dysregulated circular RNAs (circRNAs) might play vital roles in tumor development. OBJECTIVE: Here, we aimed to explore the function of a novel circRNA circ_0067772 in CSCC. METHODS: Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to determine the expression of circ_0067772, microRNA (miR)-1238-3p and forkhead box protein G1 (FOXG1). Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Transwell assay and wound healing assay were employed to examine cell metastasis. Flow cytometry was employed to monitor cell cycle and apoptosis. The target association between miR-1238-3p and circ_0067772 or FOXG1 was validated by dual-luciferase reporter assay. Moreover, role of circ_0067772 in vivo was investigated via xenograft model in nude mice. RESULTS: Circ_0067772 and FOXG1 were upregulated, while miR-1238-3p was downregulated in CSCC tissues and cells. Circ_0067772 knockdown conferred inhibitory effects on cell proliferation, migration and invasion of CSCC cells. MiR-1238-3p served as a target of circ_0067772, whose silencing could reverse circ_0067772 knockdown-induced inhibitory impact on the malignant cellular behaviors. Circ_0067772 positively regulated FOXG1 expression by antagonizing miR-1238-3p. Additionally, miR-1238-3p could repress CSCC cell proliferation, migration and invasion by targeting FOXG1. Also, circ_0067772 knockdown hindered CSCC tumor growth in vivo. CONCLUSION: Our study identified a novel oncogenic circRNA and the involvement of circ_0067772/miR-1238-3p/FOXG1 axis in CSCC development, providing a target for CSCC therapy.
Authors: D W Chan; V W S Liu; R M Y To; P M Chiu; W Y W Lee; K M Yao; A N Y Cheung; H Y S Ngan Journal: Br J Cancer Date: 2009-09-15 Impact factor: 7.640
Authors: Kunal Das Mahapatra; Lorenzo Pasquali; Jonas Nørskov Søndergaard; Jan Lapins; István Balazs Nemeth; Eszter Baltás; Lajos Kemény; Bernhard Homey; Liviu-Ionut Moldovan; Jørgen Kjems; Claudia Kutter; Enikö Sonkoly; Lasse Sommer Kristensen; Andor Pivarcsi Journal: Sci Rep Date: 2020-02-27 Impact factor: 4.379