Mouna Rajhi1,2,3, Sondes Haddad-Boubaker1,2,3, Anissa Chouikha1,2,3, Daniel Bourquain4, Janine Michel4, Walid Hammami1,2,3, Amel Sadraoui1,2,3, Hinda Touzi1,2,3, Kais Ghedira2,5, Henda Triki1,2,3. 1. Laboratory of Clinical Virology, WHO Regional Reference Laboratory for Poliomyelitis and Measles, for EMR, Pasteur Institute, Tunis, Tunisia. 2. University of Tunis El Manar, Tunis, Tunisia. 3. Research Laboratory of Virus, Vector and Host (LR20IPT10), Pasteur Institute, Tunis, Tunisia. 4. Robert Koch Institute, Centre for Biological Threats and Special Pathogens-Highly Pathogenic Viruses, Berlin, Germany. 5. Laboratory of Bioinformatics, Biomathematics and Biostatistics (LR16IPT09), Pasteur Institute, Tunis, Tunisia.
Abstract
BACKGROUND: Hepatitis C virus (HCV) has a high genetic diversity. Eight genotypes and 90 subtypes are currently described. Genotypes are clinically significant for therapeutic management and their determination is necessary for epidemiological studies. METHODS: Tunisian patients plasma samples (n = 6) with unassigned HCV-2 subtype using partial sequencing in the NS5B and Core/E1 regions were analyzed by realizing whole-genome sequencing analysis. Phylogenetic analyses were performed to assign subtypes. RESULTS: Phylogenetic analysis of the full genome sequences of Tunisian strains shows two subtypes within HCV-2. These later were genetically distinct from all previously established HCV-2 subtypes with nucleotide divergence greater than 15% (20% -31%). These two subtypes are proposed as new subtypes 2v and 2w. CONCLUSIONS: The discovery of two new HCV-2 subtypes circulating in the Tunisian population confirms the great diversity of HCV-2 viruses and increases the total number of HCV-2 subtypes from 21 to 23.
BACKGROUND:Hepatitis C virus (HCV) has a high genetic diversity. Eight genotypes and 90 subtypes are currently described. Genotypes are clinically significant for therapeutic management and their determination is necessary for epidemiological studies. METHODS: Tunisian patients plasma samples (n = 6) with unassigned HCV-2 subtype using partial sequencing in the NS5B and Core/E1 regions were analyzed by realizing whole-genome sequencing analysis. Phylogenetic analyses were performed to assign subtypes. RESULTS: Phylogenetic analysis of the full genome sequences of Tunisian strains shows two subtypes within HCV-2. These later were genetically distinct from all previously established HCV-2 subtypes with nucleotide divergence greater than 15% (20% -31%). These two subtypes are proposed as new subtypes 2v and 2w. CONCLUSIONS: The discovery of two new HCV-2 subtypes circulating in the Tunisian population confirms the great diversity of HCV-2 viruses and increases the total number of HCV-2 subtypes from 21 to 23.
Authors: K S Lole; R C Bollinger; R S Paranjape; D Gadkari; S S Kulkarni; N G Novak; R Ingersoll; H W Sheppard; S C Ray Journal: J Virol Date: 1999-01 Impact factor: 5.103
Authors: Peter V Markov; Jacques Pepin; Eric Frost; Sylvie Deslandes; Annie-Claude Labbé; Oliver G Pybus Journal: J Gen Virol Date: 2009-05-27 Impact factor: 3.891
Authors: Sajjad Ahmad; Farah Shahid; Muhammad Tahir Ul Qamar; Habib Ur Rehman; Sumra Wajid Abbasi; Wasim Sajjad; Saba Ismail; Faris Alrumaihi; Khaled S Allemailem; Ahmad Almatroudi; Hafiz Fahad Ullah Saeed Journal: Vaccines (Basel) Date: 2021-03-21