| Literature DB >> 33704749 |
Stefan Bletz1, Alexander Mellmann2, Barbara Middendorf-Bauchart1.
Abstract
Today, whole genome sequencing (WGS)-based typing is the gold standard approach to detect outbreaks of Shiga toxin-producing Escherichia coli (STEC) and to differentiate them from sporadic cases. Here, we describe an optimized protocol to efficiently determine the genome sequences of STEC using short read Illumina technology and provide information on helpful tools for the subsequent bioinformatic analysis.Entities:
Keywords: Outbreak; STEC; Shiga toxin-producing Escherichia coli; Subtyping; WGS; Whole genome sequencing
Mesh：
Year: 2021 PMID： 33704749 DOI： 10.1007/978-1-0716-1339-9_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745