Literature DB >> 33704706

A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells.

Jelena Grahovac1, Marijana Pavlović2, Marija Ostojić2.   

Abstract

Melanoma cells have high glycolytic capacity. Glucose uptake is a key rate-limiting step in glucose utilization. Here we describe a simple protocol for measuring direct glucose uptake in living melanoma cells by flow cytometry.

Entities:  

Keywords:  2-NBDG; Flow cytometry; Glucose uptake; Melanoma

Mesh：

Substances：

Year:  2021        PMID： 33704706     DOI： 10.1007/978-1-0716-1205-7_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

Review 1.  Molecular drivers of cellular metabolic reprogramming in melanoma.

Authors:  Cecilie Abildgaard; Per Guldberg
Journal:  Trends Mol Med       Date:  2015-01-21       Impact factor: 11.951

Review 2.  Molecular pathways: BRAF induces bioenergetic adaptation by attenuating oxidative phosphorylation.

Authors:  Rizwan Haq; David E Fisher; Hans R Widlund
Journal:  Clin Cancer Res       Date:  2014-03-07       Impact factor: 12.531

Review 3.  Metabolic strategies of melanoma cells: Mechanisms, interactions with the tumor microenvironment, and therapeutic implications.

Authors:  Grant M Fischer; Y N Vashisht Gopal; Jennifer L McQuade; Weiyi Peng; Ralph J DeBerardinis; Michael A Davies
Journal:  Pigment Cell Melanoma Res       Date:  2017-11-02       Impact factor: 4.693

4.  Examining glucose transport in single vascular smooth muscle cells with a fluorescent glucose analog.

Authors:  P G Lloyd; C D Hardin; M Sturek
Journal:  Physiol Res       Date:  1999       Impact factor: 1.881

5.  Intracellular fate of 2-NBDG, a fluorescent probe for glucose uptake activity, in Escherichia coli cells.

Authors:  K Yoshioka; M Saito; K B Oh; Y Nemoto; H Matsuoka; M Natsume; H Abe
Journal:  Biosci Biotechnol Biochem       Date:  1996-11       Impact factor: 2.043

6.  Metabolic targeting synergizes with MAPK inhibition and delays drug resistance in melanoma.

Authors:  Christina Brummer; Stephanie Faerber; Christina Bruss; Christian Blank; Ruben Lacroix; Sebastian Haferkamp; Wolfgang Herr; Marina Kreutz; Kathrin Renner
Journal:  Cancer Lett       Date:  2018-11-24       Impact factor: 8.679

7.  Regulation of glucose transport by pioglitazone in cultured muscle cells.

Authors:  I M el-Kebbi; S Roser; R J Pollet
Journal:  Metabolism       Date:  1994-08       Impact factor: 8.694

8.  A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli.

Authors:  K Yoshioka; H Takahashi; T Homma; M Saito; K B Oh; Y Nemoto; H Matsuoka
Journal:  Biochim Biophys Acta       Date:  1996-02-09

9.  Targeting metabolic flexibility by simultaneously inhibiting respiratory complex I and lactate generation retards melanoma progression.

Authors:  Balkrishna Chaube; Parmanand Malvi; Shivendra Vikram Singh; Naoshad Mohammad; Avtar Singh Meena; Manoj Kumar Bhat
Journal:  Oncotarget       Date:  2015-11-10

10.  Telmisartan induces melanoma cell apoptosis and synergizes with vemurafenib in vitro by altering cell bioenergetics.

Authors:  Jelena Grahovac; Tatjana Srdić-Rajić; Juan Francisco Santibañez; Marijana Pavlović; Milena Čavić; Siniša Radulović
Journal:  Cancer Biol Med       Date:  2019-05       Impact factor: 4.248
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