| Literature DB >> 33704704 |
Ewa Mazurkiewicz1, Ewa Mrówczyńska1, Aleksandra Simiczyjew1, Dorota Nowak1, Antonina J Mazur2.
Abstract
In order to protrude within a dense tissue, tumor cells have to develop the ability to digest the extracellular matrix (ECM). Melanoma cells, similarly to other types of tumor cells, form invadopodia, membranous invaginations rich in filamentous actin and several other proteins including matrix metalloproteinases (MMPs). MMPs degrade ECM structural proteins such as collagens, fibronectin, or laminin. Here we describe an assay that allows the detection of gelatinase activity exhibited by tumor cells under 2D conditions and methods to present obtained data in both a quantitative and a qualitative manner.Entities:
Keywords: Extracellular matrix (ECM) degradation; Gelatin-fluorescein degradation assay; Invadopodia; Invasion; Matrix metalloproteinases (MMPs); Melanoma
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Year: 2021 PMID： 33704704 DOI： 10.1007/978-1-0716-1205-7_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745