Literature DB >> 3369690

A partition assay for the simultaneous determination of insect juvenile hormone esterase and epoxide hydrolase activity.

M R Share1, R M Roe.   

Abstract

A partition assay was developed to measure insect juvenile hormone (JH) I and III metabolism in biological samples containing both JH esterase and JH epoxide hydrolase activity. The assay utilizes commercially available radiochain 3H-labeled JH as substrate and the selective JH esterase inhibitor 3-octylthio-1,1,1-trifluoro-2-propanone. JH partitions into an isooctane phase and the metabolites JH acid, JH diol, and JH diol-acid into aqueous methanol after incubation of JH substrate with inhibited and uninhibited sample. The assay provides a time- and cost-efficient alternative to the currently available thin-layer chromatography method for the measurement of JH esterase and epoxide hydrolase activity.

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Year:  1988        PMID： 3369690     DOI： 10.1016/0003-2697(88)90257-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

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4.  A transcriptomic atlas underlying developmental plasticity of seasonal forms of Bicyclus anynana butterflies.

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Journal:  Mol Biol Evol       Date:  2022-06-09       Impact factor: 8.800

5.  Production of epoxide hydrolases in batch fermentations of Botryosphaeria rhodina.

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6.  Identification and characterization of juvenile hormone esterase gene from the yellow fever mosquito, Aedes aegypti.

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Journal:  Insect Biochem Mol Biol       Date:  2007-05-29       Impact factor: 4.714

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8.  Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.

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Journal:  PLoS One       Date:  2014-01-29       Impact factor: 3.240

9.  Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis.

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Journal:  Sci Rep       Date:  2017-05-23       Impact factor: 4.379

10.  Dynamic changes in host-virus interactions associated with colony founding and social environment in fire ant queens (Solenopsis invicta).

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