Christopher Wilks1, Omar Ahmed1, Daniel N Baker1, David Zhang1,2,3,4, Leonardo Collado-Torres5, Ben Langmead1. 1. Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA. 2. Institute of Neurology, University College London (UCL), London, UK. 3. NIHR Great Ormond Street Hospital Biomedical Research Centre, University College London, London, UK. 4. Genetics and Genomic Medicine, Great Ormond Street Institute of Child Health, University College London, London WC1E 6BT, UK. 5. Lieber Institute for Brain Development, Baltimore, MD, USA.
Abstract
MOTIVATION: A common way to summarize sequencing datasets is to quantify data lying within genes or other genomic intervals. This can be slow and can require different tools for different input file types. RESULTS: Megadepth is a fast tool for quantifying alignments and coverage for BigWig and BAM/CRAM input files, using substantially less memory than the next-fastest competitor. Megadepth can summarize coverage within all disjoint intervals of the Gencode V35 gene annotation for more than 19,000 GTExV8 BigWig files in approximately one hour using 32 threads. Megadepth is available both as a command-line tool and as an R/Bioconductor package providing much faster quantification compared to the rtracklayer package. AVAILABILITY: https://github.com/ChristopherWilks/megadepth,https://bioconductor.org/packages/megadepth.
MOTIVATION: A common way to summarize sequencing datasets is to quantify data lying within genes or other genomic intervals. This can be slow and can require different tools for different input file types. RESULTS: Megadepth is a fast tool for quantifying alignments and coverage for BigWig and BAM/CRAM input files, using substantially less memory than the next-fastest competitor. Megadepth can summarize coverage within all disjoint intervals of the Gencode V35 gene annotation for more than 19,000 GTExV8 BigWig files in approximately one hour using 32 threads. Megadepth is available both as a command-line tool and as an R/Bioconductor package providing much faster quantification compared to the rtracklayer package. AVAILABILITY: https://github.com/ChristopherWilks/megadepth,https://bioconductor.org/packages/megadepth.
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