Chemo-enzymatic approaches for consolidated bioconversion of Saccharum spontaneum biomass to ethanol-biofuel.

A novel strategy involving sodium dodecylsulfate (SDS) (SDS assisted tris (2-hydroxyethyl) methyl- ammonium methyl sulphate ([TMA][MeSO4], ionic liquid) pretreatment of Saccharum spontaneum biomass (SSB) following its enzymatic saccharification, and conversion into ethanol-biofuel in a consolidated bioprocess (CBP) was developed. Ionic liquid stable enzyme preparation developed from Bacillus subtilis G2 was used for saccharification. Optimized pretreatment and saccharification variables enhanced the sugar yield (2.35-fold), which was fermented to ethanol content of 104.42 mg/g biomass with an efficiency of 35.73%. The pretreated biomass was examined for textural/ultrastructural alterations by scanning electron microscopy (SEM), 1H/13C nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), surface area measurements, water retention value, and cellulase adsorption isotherms. The combined [TMA][MeSO4] and SDS pretreatment disrupted the lignocellulosic microfibrils, and increased the porosity and surface area. The study provides new mechanistic insights on combined IL and surfactant pretreatment of biomass for its efficient conversion to biofuel.