Novel semi-automated graphene nanosheets based pipette-tip assisted micro-solid phase extraction as eco-friendly technique for the rapid detection of emerging environmental pollutant in waters.

In this work, a new semi-automated syringe infusion-pump assisted graphene nanosheets (GNSs) based pipette-tip micro-solid phase extraction (PT-μSPE) as a green sample preparation technique was demonstrated for the sensitive analysis of emerging environmental pollutant in environmental waters using HPLC-UV. Microwave-assisted synthesized GNSs powder was packed into a 100 μL pipette-tip (as PT-μSPE cartridge) connected with a commercial plastic syringe (contains water sample). This setup was attached to a programmable auto-syringe infusion pump for the GNSs-PT-μSPE process. Triclosan (TCS) is an emerging environmental pollutant chosen as a target analyte to examine the extraction capacity and feasibility of GNSs as a sorbent material for PT-μSPE. Parameters affecting the extraction capability were systematically evaluated and thoroughly optimized. At optimized experimental parameters, excellent linearity (r2 = 0.9979) was achieved over the concentration range of 2-250 ng mL-1 for TCS, with a detection limit of 0.5 ng mL-1. Applicability of the presented method was examined with real water samples, and extraction recoveries obtained were ranged between 94.6-102.4% with RSD less than 7.8%. The presented protocol is a simple, semi-automated, eco-friendly, low-cost, and efficient sample pretreatment technique for quick analysis of TCS in environmental wastewaters.