Literature DB >> 33689165

3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells.

Tomoko Miyake1, Mikio Shimada2.   

Abstract

The keratinocytes are predominant cells in the epidermis of the human skin. To assess the cellular response of the keratinocytes to the genotoxic stress, we derived the skin keratinocytes from human induced pluripotent stem cells (iPSCs). Furthermore, three-dimensional (3D) organoid culture method is powerful tool to analyze the organ and tissue response against the genotoxic stress. Here we describe the method of 3D organoid culture using skin keratinocytes derived from human iPSCs.
© 2021. Springer Science+Business Media, LLC.

Entities:  

Keywords:  3D organoid culture; Epidermis; Genotoxic stress; Human iPS cells; Skin keratinocytes

Mesh：

Year:  2022        PMID： 33689165     DOI： 10.1007/7651_2021_357

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Evidence-based skin care management in radiation therapy: clinical update.

Authors:  Maurene McQuestion
Journal:  Semin Oncol Nurs       Date:  2011-05       Impact factor: 2.315

2.  Differentiation of mouse induced pluripotent stem cells into a multipotent keratinocyte lineage.

Authors:  Ganna Bilousova; Jiang Chen; Dennis R Roop
Journal:  J Invest Dermatol       Date:  2010-12-09       Impact factor: 8.551

3.  Genotoxic stress abrogates renewal of melanocyte stem cells by triggering their differentiation.

Authors:  Ken Inomata; Takahiro Aoto; Nguyen Thanh Binh; Natsuko Okamoto; Shintaro Tanimura; Tomohiko Wakayama; Shoichi Iseki; Eiji Hara; Takuji Masunaga; Hiroshi Shimizu; Emi K Nishimura
Journal:  Cell       Date:  2009-06-12       Impact factor: 41.582

4.  Global gene expression responses to low- or high-dose radiation in a human three-dimensional tissue model.

Authors:  Alexandre Mezentsev; Sally A Amundson
Journal:  Radiat Res       Date:  2011-04-12       Impact factor: 2.841

5.  DNA Damage Response After Ionizing Radiation Exposure in Skin Keratinocytes Derived from Human-Induced Pluripotent Stem Cells.

Authors:  Tomoko Miyake; Mikio Shimada; Yoshihisa Matsumoto; Akitoshi Okino
Journal:  Int J Radiat Oncol Biol Phys       Date:  2019-05-11       Impact factor: 7.038

6.  Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

Authors:  Kazutoshi Takahashi; Shinya Yamanaka
Journal:  Cell       Date:  2006-08-10       Impact factor: 41.582

7.  Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo.

Authors:  Ophelia Veraitch; Tetsuro Kobayashi; Yoichi Imaizumi; Wado Akamatsu; Takashi Sasaki; Shinya Yamanaka; Masayuki Amagai; Hideyuki Okano; Manabu Ohyama
Journal:  J Invest Dermatol       Date:  2013-01-15       Impact factor: 8.551

8.  Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Authors:  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka
Journal:  Cell       Date:  2007-11-30       Impact factor: 41.582

9.  Retinoic acid and bone morphogenetic protein signaling synergize to efficiently direct epithelial differentiation of human embryonic stem cells.

Authors:  Christian M Metallo; Lin Ji; Juan J de Pablo; Sean P Palecek
Journal:  Stem Cells       Date:  2007-10-25       Impact factor: 6.277

10.  Induction of hair follicle dermal papilla cell properties in human induced pluripotent stem cell-derived multipotent LNGFR(+)THY-1(+) mesenchymal cells.

Authors:  Ophelia Veraitch; Yo Mabuchi; Yumi Matsuzaki; Takashi Sasaki; Hironobu Okuno; Aki Tsukashima; Masayuki Amagai; Hideyuki Okano; Manabu Ohyama
Journal:  Sci Rep       Date:  2017-02-21       Impact factor: 4.379
View more
  1 in total

1.  Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.

Authors:  Yunyao Bo; Lijun Yang; Baiting Liu; Guiping Tian; Chenxi Li; Lin Zhang; Yuan Yan
Journal:  J Nanobiotechnology       Date:  2022-06-21       Impact factor: 9.429
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.