Literature DB >> 33689076

The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties.

A Yu Gorokhovatsky1, T V Chepurnykh1, A S Shcheglov1, Yu A Mokrushina1, M N Baranova1, S A Goncharuk1, K V Purtov2, V N Petushkov3, N S Rodionova3, I V Yampolsky1.   

Abstract

A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase.

Entities:  

Keywords:  Neonothopanus nambi; Pichia pastoris; bioluminescence; heterologous expression; luciferase; nnLuz

Mesh：

Substances：

Year:  2021        PMID： 33689076      PMCID： PMC7946651          DOI： 10.1134/S1607672921010051

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  6 in total

Review 1.  Luminescence-based Imaging Approaches in the Field of Interventional Molecular Imaging.

Authors:  Fijs W B van Leeuwen; James C H Hardwick; Arian R van Erkel
Journal:  Radiology       Date:  2015-07       Impact factor: 11.105

Review 2.  NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence.

Authors:  Christopher G England; Emily B Ehlerding; Weibo Cai
Journal:  Bioconjug Chem       Date:  2016-04-19       Impact factor: 4.774

3.  Genetically encodable bioluminescent system from fungi.

Authors:  Alexey A Kotlobay; Karen S Sarkisyan; Yuliana A Mokrushina; Marina Marcet-Houben; Ekaterina O Serebrovskaya; Nadezhda M Markina; Louisa Gonzalez Somermeyer; Andrey Y Gorokhovatsky; Andrey Vvedensky; Konstantin V Purtov; Valentin N Petushkov; Natalja S Rodionova; Tatiana V Chepurnyh; Liliia I Fakhranurova; Elena B Guglya; Rustam Ziganshin; Aleksandra S Tsarkova; Zinaida M Kaskova; Victoria Shender; Maxim Abakumov; Tatiana O Abakumova; Inna S Povolotskaya; Fedor M Eroshkin; Andrey G Zaraisky; Alexander S Mishin; Sergey V Dolgov; Tatiana Y Mitiouchkina; Eugene P Kopantzev; Hans E Waldenmaier; Anderson G Oliveira; Yuichi Oba; Ekaterina Barsova; Ekaterina A Bogdanova; Toni Gabaldón; Cassius V Stevani; Sergey Lukyanov; Ivan V Smirnov; Josef I Gitelson; Fyodor A Kondrashov; Ilia V Yampolsky
Journal:  Proc Natl Acad Sci U S A       Date:  2018-11-26       Impact factor: 11.205

Review 4.  Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy: In Vitro and In Vivo Perspectives.

Authors:  Yongcun Yan; Pengfei Shi; Weiling Song; Sai Bi
Journal:  Theranostics       Date:  2019-05-31       Impact factor: 11.556

Review 5.  A brief review of bioluminescent systems (2019).

Authors:  Aubin Fleiss; Karen S Sarkisyan
Journal:  Curr Genet       Date:  2019-03-08       Impact factor: 3.886

6.  Plants with genetically encoded autoluminescence.

Authors:  Tatiana Mitiouchkina; Alexander S Mishin; Louisa Gonzalez Somermeyer; Nadezhda M Markina; Ilia V Yampolsky; Karen S Sarkisyan; Tatiana V Chepurnyh; Elena B Guglya; Tatiana A Karataeva; Kseniia A Palkina; Ekaterina S Shakhova; Liliia I Fakhranurova; Sofia V Chekova; Aleksandra S Tsarkova; Yaroslav V Golubev; Vadim V Negrebetsky; Sergey A Dolgushin; Pavel V Shalaev; Dmitry Shlykov; Olesya A Melnik; Victoria O Shipunova; Sergey M Deyev; Andrey I Bubyrev; Alexander S Pushin; Vladimir V Choob; Sergey V Dolgov; Fyodor A Kondrashov
Journal:  Nat Biotechnol       Date:  2020-04-27       Impact factor: 54.908
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.