Soad A Mohamad1, Eman Maher Zahran2, Maha Raafat Abdel Fadeel3, Amgad Albohy4, Mohamed A Safwat5. 1. Department of Pharmaceutics, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt. 2. Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt. 3. Veterinary Serum and Vaccine Research Institute (VSVRI), Cairo, Egypt. 4. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The British University in Egypt (BUE), El-Sherouk City, 1837, Egypt. 5. Department of Pharmaceutics, Faculty of Pharmacy, South Valley University, Qena, 83523, Egypt.
Abstract
BACKGROUND: SARS-COVID-2 has recently been one of the most life-threatening problems which urgently needs new therapeutic antiviral agents, especially those of herbal origin. PURPOSE: The study aimed to load acaciin (ACA) into the new self-assembled nanofibers (NFs) followed by investigating their possible antiviral effect against bovine coronavirus (BCV) as a surrogate model for SARS-COV-2. METHODS: ACA was identified using 1H-NMR and DEPT-Q 13C-NMR spectroscopy, the molecular docking study was performed using Autodock 4 and a modification of the traditional solvent injection method was applied for the synthesis of the biodegradable NFs. Different characterization techniques were used to inspect the formation of the NFs, which is followed by antiviral investigation against BCV as well as MTT assay using MDBK cells. RESULTS: Core/shell NFs, ranging between 80-330 nm with tiny thorn-like branches, were formed which attained an enhanced encapsulation efficiency (97.5 ± 0.53%, P<0.05) and a dual controlled release (a burst release of 65% at 1 h and a sustained release up to >24 h). The antiviral investigation of the formed NFs revealed a significant inhibition of 98.88 ± 0.16% (P<0.05) with IC50 of 12.6 µM against BCV cells. CONCLUSION: The results introduced a new, time/cost-saving strategy for the synthesis of biodegradable NFs without the need for electric current or hazardous cross-linking agents. Moreover, it provided an innovative avenue for the discovery of drugs of herbal origin for the fight against SARS-CoV-2 infection.
BACKGROUND: SARS-COVID-2 has recently been one of the most life-threatening problems which urgently needs new therapeutic antiviral agents, especially those of herbal origin. PURPOSE: The study aimed to load acaciin (ACA) into the new self-assembled nanofibers (NFs) followed by investigating their possible antiviral effect against bovine coronavirus (BCV) as a surrogate model for SARS-COV-2. METHODS: ACA was identified using 1H-NMR and DEPT-Q 13C-NMR spectroscopy, the molecular docking study was performed using Autodock 4 and a modification of the traditional solvent injection method was applied for the synthesis of the biodegradable NFs. Different characterization techniques were used to inspect the formation of the NFs, which is followed by antiviral investigation against BCV as well as MTT assay using MDBK cells. RESULTS: Core/shell NFs, ranging between 80-330 nm with tiny thorn-like branches, were formed which attained an enhanced encapsulation efficiency (97.5 ± 0.53%, P<0.05) and a dual controlled release (a burst release of 65% at 1 h and a sustained release up to >24 h). The antiviral investigation of the formed NFs revealed a significant inhibition of 98.88 ± 0.16% (P<0.05) with IC50 of 12.6 µM against BCV cells. CONCLUSION: The results introduced a new, time/cost-saving strategy for the synthesis of biodegradable NFs without the need for electric current or hazardous cross-linking agents. Moreover, it provided an innovative avenue for the discovery of drugs of herbal origin for the fight against SARS-CoV-2 infection.
Authors: Usama Ramadan Abdelmohsen; Amgad Albohy; Basma S Abdulrazik; Soad A L Bayoumi; Lourin G Malak; Iman S A Khallaf; Gerhard Bringmann; Salwa F Farag Journal: RSC Adv Date: 2021-05-07 Impact factor: 4.036
Authors: Eman Maher Zahran; Ahmed M Sayed; Miada F Abdelwahab; Amgad Albohy; Basma S Abdulrazik; Ayman M Ibrahim; Gerhard Bringmann; Usama Ramadan Abdelmohsen Journal: RSC Adv Date: 2021-11-18 Impact factor: 4.036
Authors: Ivana Jarak; Inês Silva; Cátia Domingues; Ana Isabel Santos; Francisco Veiga; Ana Figueiras Journal: Int J Mol Sci Date: 2022-08-02 Impact factor: 6.208
Authors: Abeer H Elmaidomy; Eman Maher Zahran; Raya Soltane; Ahlam Alasiri; Hani Saber; Che Julius Ngwa; Gabriele Pradel; Faisal Alsenani; Ahmed M Sayed; Usama Ramadan Abdelmohsen Journal: Molecules Date: 2022-08-31 Impact factor: 4.927