Preliminary observations on the effects in vivo and in vitro of low dose laser on the epithelia of the bladder, trachea and tongue of the mouse.

The effects of low dose CW laser were studied by in vivo and in vitro systems. The experimental tissues that were used included bladders, tracheas and tongues as experimental tissues. Buddings (round surface projections) from the transitional epithelium of bladder were frequently observed 3 days after laser treatment in both in vivo and in vitro systems. The trachea and tongue were less affected. In both the in vivo and in vitro systems, some epithelial cells of the trachea showed decreased microvilli and cilia 3 days after treatment whereas the epithelial cells of the tongue revealed no response to laser treatment in both systems. Low dose laser, however, appeared to promote the rate of healing of experimental tongue ulcer: healing was about 1 day earlier in the laser treated than non-treated animals and vessel infiltration and epithelialization were detected earlier in the treated.