| Literature DB >> 33687705 |
Miriam Morente-López1, Juan A Fafián-Labora1, Mónica Carrera2, Francisco J de Toro1, Concha Gil3, Jesús Mateos4, María C Arufe5.
Abstract
In the present protocol, extracellular vesicles (EVs) released from a primary culture of human umbilical cord mesenchymal stem cells (MSCs) were isolated by ultracentrifugation processes, characterized by transmission electron microscopy (TEM) and measured by nanoparticle tracking analysis (NTA). Protein was extracted from EVs using RIPA buffer and then was assessed for integrity. The proteomic content of the total EV protein samples was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after labeling by tandem mass tag (TMT). This combined approach allowed the development of an effective strategy to study the protein cargo from MSC-derived EVs.Entities:
Keywords: Extracellular vesicles; Mesenchymal stem cells; Tandem mass tag; Transmission electron microscopy
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Year: 2021 PMID： 33687705 DOI： 10.1007/978-1-0716-1178-4_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745