| Literature DB >> 33687673 |
Ying-Wei Lan1, Chuan-Mu Chen2,3, Kowit-Yu Chong4,5,6.
Abstract
A co-culture model of mesenchymal stem cells (MSCs) and fibroblasts is an efficient and rapid method to evaluate the anti-fibrotic effects of MSCs-based cell therapy. Transforming growth factor (TGF)-β1 plays a key role in promotion of fibroblast activation and differentiation which can induce collagen deposition, increase ECM production in lung tissue, eventually resulted in pulmonary fibrosis. Here, we use this co-culture system and examine the ECM production in activated fibroblasts by western blot and quantitative real-time analysis to understand the therapeutic effects of MSCs.Entities:
Keywords: Co-culture; Conditioned medium; Extracellular matrix; Pulmonary fibrosis; TGF-β1
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Year: 2021 PMID： 33687673 DOI： 10.1007/978-1-0716-1225-5_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745