Sameh Samir Ali1,2, Rania Al-Tohamy3, Eleni Koutra4,5, Michael Kornaros4,5, Maha Khalil6,7, Tamer Elsamahy3, Mohamed El-Shetehy6,8, Jianzhong Sun9. 1. Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013, China. samh@ujs.edu.cn. 2. Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt. samh@ujs.edu.cn. 3. Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013, China. 4. Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, 1 Karatheodori Str., University Campus, 26504, Patras, Greece. 5. INVALOR: Research Infrastructure for Waste Valorization and Sustainable Management, University Campus, 26504, Patras, Greece. 6. Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt. 7. Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia. 8. Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. 9. Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013, China. jzsun1002@ujs.edu.cn.
Abstract
BACKGROUND: Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. However, an effective and environmentally friendly treatment method has not yet been implemented, while concurrently, the increasing demand of modern societies for adequate and sustainable energy supply still remains a global challenge. Under this scope, the purpose of the present study was to isolate promising species of yeasts inhabiting wood-feeding termite guts, for combined azo dyes and textile wastewater bioremediation, along with biodiesel production. RESULTS: Thirty-eight yeast strains were isolated, molecularly identified and subsequently tested for desired enzymatic activity, lipid accumulation, and tolerance to lignin-derived metabolites. The most promising species were then used for construction of a novel yeast consortium, which was further evaluated for azo dyes degradation, under various culture conditions, dye levels, as well as upon the addition of heavy metals, different carbon and nitrogen sources, and lastly agro-waste as an inexpensive and environmentally friendly substrate alternative. The novel yeast consortium, NYC-1, which was constructed included the manganese-dependent peroxidase producing oleaginous strains Meyerozyma caribbica, Meyerozyma guilliermondii, Debaryomyces hansenii, and Vanrija humicola, and showed efficient azo dyes decolorization, which was further enhanced depending on the incubation conditions. Furthermore, enzymatic activity, fatty acid profile and biodiesel properties were thoroughly investigated. Lastly, a dye degradation pathway coupled to biodiesel production was proposed, including the formation of phenol-based products, instead of toxic aromatic amines. CONCLUSION: In total, this study might be the first to explore the application of MnP and lipid-accumulating yeasts for coupling dye degradation and biodiesel production.
BACKGROUND: Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. However, an effective and environmentally friendly treatment method has not yet been implemented, while concurrently, the increasing demand of modern societies for adequate and sustainable energy supply still remains a global challenge. Under this scope, the purpose of the present study was to isolate promising species of yeasts inhabiting wood-feeding termite guts, for combined azo dyes and textile wastewater bioremediation, along with biodiesel production. RESULTS: Thirty-eight yeast strains were isolated, molecularly identified and subsequently tested for desired enzymatic activity, lipid accumulation, and tolerance to lignin-derived metabolites. The most promising species were then used for construction of a novel yeast consortium, which was further evaluated for azo dyes degradation, under various culture conditions, dye levels, as well as upon the addition of heavy metals, different carbon and nitrogen sources, and lastly agro-waste as an inexpensive and environmentally friendly substrate alternative. The novel yeast consortium, NYC-1, which was constructed included the manganese-dependent peroxidase producing oleaginous strains Meyerozyma caribbica, Meyerozyma guilliermondii, Debaryomyces hansenii, and Vanrija humicola, and showed efficient azo dyes decolorization, which was further enhanced depending on the incubation conditions. Furthermore, enzymatic activity, fatty acid profile and biodiesel properties were thoroughly investigated. Lastly, a dye degradation pathway coupled to biodiesel production was proposed, including the formation of phenol-based products, instead of toxic aromatic amines. CONCLUSION: In total, this study might be the first to explore the application of MnP and lipid-accumulating yeasts for coupling dye degradation and biodiesel production.
Authors: Chandrakant R Holkar; Ananda J Jadhav; Dipak V Pinjari; Naresh M Mahamuni; Aniruddha B Pandit Journal: J Environ Manage Date: 2016-08-03 Impact factor: 6.789
Authors: Muhammad Imran; Baby Shaharoona; David E Crowley; Azeem Khalid; Sabir Hussain; Muhammad Arshad Journal: Ecotoxicol Environ Saf Date: 2015-06-11 Impact factor: 6.291
Authors: Sameh Samir Ali; Rania Al-Tohamy; Eleni Koutra; Amal H El-Naggar; Michael Kornaros; Jianzhong Sun Journal: J Hazard Mater Date: 2020-07-29 Impact factor: 10.588