C M C Volgenant1, M A Hoogenkamp1, G Dahlén2, S Kalfas3, S Petti4, J J De Soet1. 1. Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands. 2. Department of Public Health and Infectious Diseases, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Sweden. 3. Division of Preventive Dentistry Periodontology and Implant Biology, Aristotle University of Thessaloniki, Thessaloniki Greece. 4. Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy.
Abstract
Objective: This study assessed the prevalence of MRSA, ESBL and VRE in students from four dental schools in Europe. Methods: The hand, tongue and nostrils of the students who treated patients (study group) and who did not treat patients (control group) were sampled. After incubation in TSB and subculturing in the presence of 4 µg/ml oxacillin, positive cultures were identified for Staphylococcus aureus by Mannitol salt agar and agglutination tests. The presence of MRSA was confirmed by specific PCR on the species and on the SSCmec genes. ESBL and VRE were isolated using specific CHROMagar and confirmed using antibiotic sensitivity tests. Results: Of the 879 students who participated in this study (454 students which treated patients, 425 controls) a total of 50 students (5.7%) tested positive for a multi-drug resistant bacterium (MDRB); 13 (1.5%) students tested positive for MRSA, 26 (3.0%) for ESBL and 12 (1.4%) for VRE. No statistically significant differences were found between the students who treated patients compared to the control group for any of the MDRB and study centres, excluding MRSA carriage in the Italian student population. The use of antibiotics the year before sampling, was positively associated with the presence of an MDRB (OR 2.0; 95% Confidence Interval 1.10-3.68; p = 0.02). Conclusion: The risk for MDRB carriage and sequential transmission of MDRB for dental health care students and their patients were acceptably low.
Objective: This study assessed the prevalence of MRSA, ESBL and VRE in students from four dental schools in Europe. Methods: The hand, tongue and nostrils of the students who treated patients (study group) and who did not treat patients (control group) were sampled. After incubation in TSB and subculturing in the presence of 4 µg/ml oxacillin, positive cultures were identified for Staphylococcus aureus by Mannitol salt agar and agglutination tests. The presence of MRSA was confirmed by specific PCR on the species and on the SSCmec genes. ESBL and VRE were isolated using specific CHROMagar and confirmed using antibiotic sensitivity tests. Results: Of the 879 students who participated in this study (454 students which treated patients, 425 controls) a total of 50 students (5.7%) tested positive for a multi-drug resistant bacterium (MDRB); 13 (1.5%) students tested positive for MRSA, 26 (3.0%) for ESBL and 12 (1.4%) for VRE. No statistically significant differences were found between the students who treated patients compared to the control group for any of the MDRB and study centres, excluding MRSA carriage in the Italian student population. The use of antibiotics the year before sampling, was positively associated with the presence of an MDRB (OR 2.0; 95% Confidence Interval 1.10-3.68; p = 0.02). Conclusion: The risk for MDRB carriage and sequential transmission of MDRB for dental health care students and their patients were acceptably low.
Authors: F J Martínez-Ruíz; T Y Carrillo-Espíndola; J Bustos-Martínez; A Hamdan-Partida; L Sánchez-Pérez; A E Acosta-Gío Journal: J Hosp Infect Date: 2014-01-14 Impact factor: 3.926
Authors: Sotirios Tsiodras; George L Daikos; Andie Lee; Diamantis Plachouras; Anastasia Antoniadou; Fani Ploiarchopoulou; Mina Psichogiou; George Petrikkos; Stephan Harbarth Journal: J Glob Antimicrob Resist Date: 2013-10-26 Impact factor: 4.035
Authors: Claudia Peters; Madeleine Dulon; Albert Nienhaus; Anja Schablon Journal: Int J Environ Res Public Health Date: 2019-06-04 Impact factor: 3.390