Fangfang Chen1, Shuai Yin2, Bin Luo1, Xiaoyan Wu1, Honglin Yan1, Dandan Yan1, Chuang Chen3, Feng Guan1, Jingping Yuan1. 1. Department of Pathology, Renmin Hospital of Wuhan University, No. 99, Zhangzhidong Road, Wuchang District, Wuhan, 430060 Hubei, China. 2. Department of Gastroenterology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei, China. 3. Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060 Hubei, China.
Abstract
AIM: The main objective of this article was to evaluate the association of voltage-dependent anion channel 1 (VDAC1) with Cytochrome C (Cytc) expression, various clinicopathological features, and prognosis in breast cancer (BC) patients. Meanwhile, the correlation of Cytc expression with various clinical features and 5-year disease-free survival (5-DFS) of BC was also investigated. METHODS: In vivo, expression of VDAC1 and Cytc was examined in 219 BC tissues and 100 benign breast lesions by immunohistochemical (IHC) analysis. In vitro, MTT and wound healing migration assay were performed to detect the effect of VDAC1 on BC cells. RESULTS: Expression of VDAC1 is conversely associated with Cytc in BC (P = 0.011), especially in triple-negative breast cancer (TNBC) (P = 0.004). Knockdown of VDAC1 inhibited proliferation (P < 0.001) and migration (P < 0.05) of MCF-7 cells. High expression of VDAC1 and low expression of Cytc had a significant association with multiple clinicopathological parameters (P < 0.05) and poor 5-DFS (P < 0.001) in BC. CONCLUSION: VDAC1 was elevated in BC tissues and conversely associated with Cytc. Detection of VDAC1 may provide guidance for the poor prognosis of BC, especially TNBC.
AIM: The main objective of this article was to evaluate the association of voltage-dependent anion channel 1 (VDAC1) with Cytochrome C (Cytc) expression, various clinicopathological features, and prognosis in breast cancer (BC) patients. Meanwhile, the correlation of Cytc expression with various clinical features and 5-year disease-free survival (5-DFS) of BC was also investigated. METHODS: In vivo, expression of VDAC1 and Cytc was examined in 219 BC tissues and 100 benign breast lesions by immunohistochemical (IHC) analysis. In vitro, MTT and wound healing migration assay were performed to detect the effect of VDAC1 on BC cells. RESULTS: Expression of VDAC1 is conversely associated with Cytc in BC (P = 0.011), especially in triple-negative breast cancer (TNBC) (P = 0.004). Knockdown of VDAC1 inhibited proliferation (P < 0.001) and migration (P < 0.05) of MCF-7 cells. High expression of VDAC1 and low expression of Cytc had a significant association with multiple clinicopathological parameters (P < 0.05) and poor 5-DFS (P < 0.001) in BC. CONCLUSION: VDAC1 was elevated in BC tissues and conversely associated with Cytc. Detection of VDAC1 may provide guidance for the poor prognosis of BC, especially TNBC.
Authors: Carol E DeSantis; Jiemin Ma; Mia M Gaudet; Lisa A Newman; Kimberly D Miller; Ann Goding Sauer; Ahmedin Jemal; Rebecca L Siegel Journal: CA Cancer J Clin Date: 2019-10-02 Impact factor: 508.702
Authors: Mostafa A Aboouf; Julia Armbruster; Markus Thiersch; Franco Guscetti; Glen Kristiansen; Peter Schraml; Anne Bicker; Ruben Petry; Thomas Hankeln; Max Gassmann; Thomas A Gorr Journal: Int J Mol Sci Date: 2022-09-29 Impact factor: 6.208