Literature DB >> 3368027

Preparation and purification of gamma gamma enolase (neuron-specific enolase) using high performance anion exchange chromatography.

J L Viallard1, M R Ven Murthy, B Dastugue.   

Abstract

A simple and rapid method, using only two chromatographic steps, is described for the purification and preparation of gamma gamma enolase isoenzymes from human and beef brain extracts. In the first step, a crude gamma gamma enolase was obtained by chromatography on Q-Sepharose Fast Flow column. The crude fraction was then purified by high performance anion exchange chromatography on a Mono-Q column. gamma gamma enolase obtained in this manner was shown to be homogeneous by two dimensional polyacrylamide gel electrophoresis and by high performance gel permeation chromatography. The yield of gamma gamma enolase by this method was 7-8 mg of pure enzyme per 100 g of brain.

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Year:  1988        PMID： 3368027     DOI： 10.1007/bf00971851

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  16 in total

1.  Creatine kinase in serum: 1. Determination of optimum reaction conditions.

Authors:  G Szasz; W Gruber; E Bernt
Journal:  Clin Chem       Date:  1976-05       Impact factor: 8.327

2.  Demonstration of serum creatine kinase isoenzymes by fluorescence technique.

Authors:  H Somer; A Konttinen
Journal:  Clin Chim Acta       Date:  1972-08       Impact factor: 3.786

3.  Preparation and properties of the brain specific protein 14-3-2.

Authors:  A Grasso; G Roda; R A Hogue-Angeletti; B W Moore; V J Perez
Journal:  Brain Res       Date:  1977-04-01       Impact factor: 3.252

4.  Biochemical and immunological properties of the mouse brain enolases purified by a simple method.

Authors:  A Keller; H Scarna; A Mermet; J F Pujol
Journal:  J Neurochem       Date:  1981-04       Impact factor: 5.372

5.  Rapid electrophoretic determination of neuron-specific enolase isoenzymes in serum.

Authors:  J L Viallard; M R Ven Murthy; B Dastugue
Journal:  Clin Chem       Date:  1986-04       Impact factor: 8.327

6.  Isolation and characterization of the nervous system-specific protein 14-3-2 from rat brain. Purification, subunit composition, and comparison to the beef brain protein.

Authors:  P J Marangos; C Zomzely-Neurath; D C Luk; C York
Journal:  J Biol Chem       Date:  1975-03-10       Impact factor: 5.157

7.  Rat brain enolase isozymes. Purification of three forms of enolase.

Authors:  F Suzuki; Y Umeda; K Kato
Journal:  J Biochem       Date:  1980-06       Impact factor: 3.387

8.  Purification, radioimmunoassay, and distribution of human brain 14-3-2 protein (nervous-system specific enolase) in human tissues.

Authors:  D A Hullin; K Brown; P A Kynoch; C Smith; R J Thompson
Journal:  Biochim Biophys Acta       Date:  1980-02-21

9.  Raised neuron-specific enolase in serum of children with metastatic neuroblastoma. A report from the Children's Cancer Study Group.

Authors:  P M Zeltzer; P J Marangos; A M Parma; H Sather; A Dalton; D Hammond; S E Siegel; R C Seeger
Journal:  Lancet       Date:  1983-08-13       Impact factor: 79.321

10.  Neuron-specific enolase and S-100 protein levels in cerebrospinal fluid of patients with various neurological diseases.

Authors:  K Mokuno; K Kato; K Kawai; Y Matsuoka; T Yanagi; I Sobue
Journal:  J Neurol Sci       Date:  1983 Aug-Sep       Impact factor: 3.181
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  1 in total

1.  Repeated electroconvulsive shock selectively increases the expression of the neuron specific enolase in piriform cortex.

Authors:  C V Rasmussen; J Kragh; T G Bolwig; O S Jørgensen
Journal:  Neurochem Res       Date:  1994-12       Impact factor: 3.996
  1 in total

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