Ying Wang1, Yinhui Yao1, Jingyi Zhao2, Chunhua Cai3, Junhui Hu1, Yanwu Zhao1. 1. Department of Pharmacy, The Affiliated Hospital of Chengde Medical College, Chengde 067000, China. 2. Department of Functional Center, Chengde Medical College, Chengde 067000, China. 3. Department of Medical Insurance, The Affiliated Hospital of Chengde Medical College, Chengde 067000, China.
Abstract
BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is a fatal malignancy of the urinary system. Autophagy is implicated in KIRC occurrence and development. Here, we evaluated the prognostic value of autophagy-related genes (ARGs) in kidney renal clear cell carcinoma. MATERIALS AND METHODS: We analyzed RNA sequencing and clinical KIRC patient data obtained from TCGA and ICGC to develop an ARG prognostic signature. Differentially expressed ARGs were further evaluated by functional assessment and bioinformatic analysis. Next, ARG score was determined in 215 KIRC patients using univariable Cox and LASSO regression analyses. An ARG nomogram was built based on multivariable Cox analysis. The prognosis nomogram model based on the ARG signatures and clinicopathological information was evaluated for discrimination, calibration, and clinical usefulness. RESULTS: A total of 47 differentially expressed ARGs were identified. Of these, 8 candidates that significantly correlated with KIRC overall survival were subjected to LASSO analysis and an ARG score built. Functional enrichment and bioinformatic analysis were used to reveal the differentially expressed ARGs in cancer-related biological processes and pathways. Multivariate Cox analysis was used to integrate the ARG nomogram with the ARG signature and clinicopathological information. The nomogram exhibited proper calibration and discrimination (C-index = 0.75, AUC = >0.7). Decision curve analysis also showed that the nomogram was clinically useful. CONCLUSIONS: KIRC patients and doctors could benefit from ARG nomogram use in clinical practice.
BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is a fatal malignancy of the urinary system. Autophagy is implicated in KIRC occurrence and development. Here, we evaluated the prognostic value of autophagy-related genes (ARGs) in kidney renal clear cell carcinoma. MATERIALS AND METHODS: We analyzed RNA sequencing and clinical KIRC patient data obtained from TCGA and ICGC to develop an ARG prognostic signature. Differentially expressed ARGs were further evaluated by functional assessment and bioinformatic analysis. Next, ARG score was determined in 215 KIRC patients using univariable Cox and LASSO regression analyses. An ARG nomogram was built based on multivariable Cox analysis. The prognosis nomogram model based on the ARG signatures and clinicopathological information was evaluated for discrimination, calibration, and clinical usefulness. RESULTS: A total of 47 differentially expressed ARGs were identified. Of these, 8 candidates that significantly correlated with KIRC overall survival were subjected to LASSO analysis and an ARG score built. Functional enrichment and bioinformatic analysis were used to reveal the differentially expressed ARGs in cancer-related biological processes and pathways. Multivariate Cox analysis was used to integrate the ARG nomogram with the ARG signature and clinicopathological information. The nomogram exhibited proper calibration and discrimination (C-index = 0.75, AUC = >0.7). Decision curve analysis also showed that the nomogram was clinically useful. CONCLUSIONS: KIRC patients and doctors could benefit from ARG nomogram use in clinical practice.
Authors: Rafael Bejar; Kristen E Stevenson; Bennett A Caughey; Omar Abdel-Wahab; David P Steensma; Naomi Galili; Azra Raza; Hagop Kantarjian; Ross L Levine; Donna Neuberg; Guillermo Garcia-Manero; Benjamin L Ebert Journal: J Clin Oncol Date: 2012-08-06 Impact factor: 44.544
Authors: Leticia Serrano-Oviedo; Marta Ortega-Muelas; Jesús García-Cano; María Ll Valero; Francisco J Cimas; Raquel Pascual-Serra; Diego M Fernandez-Aroca; Olga Roche; María J Ruiz-Hidalgo; Borja Belandia; José M Giménez-Bachs; Antonio S Salinas; Ricardo Sanchez-Prieto Journal: PLoS One Date: 2018-07-26 Impact factor: 3.240