Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in healthy adults. In newborns, it can occasionally lead to a spectrum of malformations, the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues, we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to the ZIKV during pregnancy in the 2016-2017 Zika outbreak, who gave birth to infants affected by neurological complications or asymptomatic ones. Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools (CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed an increased CD4 and CD8 T-cell responses in mothers compared to children. The degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers, and children, while in CD8 T-cells, low responses were detected in these study groups. The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4 ZIKV MP. Conclusion: Donors with a history of ZIKV infection demonstrated long-term CD4 T cell immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities of CD8 T cells are markedly demonstrated in the early stages of infection, but less detected in the disease resolution phase, when the virus has already been eliminated. The responses of mothers' T cells to ZIKV MPs do not appear to be related to their children's clinical outcome. There was also no marked difference in the T cell responses to ZIKV MP between children affected or not with CZS. These data still need to be investigated, including the evaluation of the response of CD8 T cells to other ZIKV peptides.
Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in healthy adults. In newborns, it can occasionally lead to a spectrum of malformations, the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a history of ZIKVinfection were able to develop long-lasting T-cell immunity. To these issues, we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to the ZIKV during pregnancy in the 2016-2017 Zika outbreak, who gave birth to infants affected by neurological complications or asymptomatic ones. Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell responses for flow cytometry assays in response to CD4ZIKV and CD8ZIKV megapools (CD4ZIKV MP and CD8ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed an increased CD4 and CD8 T-cell responses in mothers compared to children. The degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers, and children, while in CD8 T-cells, low responses were detected in these study groups. The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4ZIKV MP. Conclusion: Donors with a history of ZIKVinfection demonstrated long-term CD4 T cell immunity to ZIKVCD4 MP. However, the same was not observed in CD8 T cells with the ZIKVCD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities of CD8 T cells are markedly demonstrated in the early stages of infection, but less detected in the disease resolution phase, when the virus has already been eliminated. The responses of mothers' T cells to ZIKV MPs do not appear to be related to their children's clinical outcome. There was also no marked difference in the T cell responses to ZIKV MP between children affected or not with CZS. These data still need to be investigated, including the evaluation of the response of CD8 T cells to other ZIKV peptides.
Authors: Jernej Mlakar; Misa Korva; Nataša Tul; Mara Popović; Mateja Poljšak-Prijatelj; Jerica Mraz; Marko Kolenc; Katarina Resman Rus; Tina Vesnaver Vipotnik; Vesna Fabjan Vodušek; Alenka Vizjak; Jože Pižem; Miroslav Petrovec; Tatjana Avšič Županc Journal: N Engl J Med Date: 2016-02-10 Impact factor: 91.245
Authors: Fernanda R Cugola; Isabella R Fernandes; Fabiele B Russo; Beatriz C Freitas; João L M Dias; Katia P Guimarães; Cecília Benazzato; Nathalia Almeida; Graciela C Pignatari; Sarah Romero; Carolina M Polonio; Isabela Cunha; Carla L Freitas; Wesley N Brandão; Cristiano Rossato; David G Andrade; Daniele de P Faria; Alexandre T Garcez; Carlos A Buchpigel; Carla T Braconi; Erica Mendes; Amadou A Sall; Paolo M de A Zanotto; Jean Pierre S Peron; Alysson R Muotri; Patricia C B Beltrão-Braga Journal: Nature Date: 2016-05-11 Impact factor: 49.962
Authors: Ryan D Pardy; Maaran M Rajah; Stephanie A Condotta; Nathan G Taylor; Selena M Sagan; Martin J Richer Journal: PLoS Pathog Date: 2017-02-23 Impact factor: 6.823
Authors: Katja Steinhagen; Christian Probst; Christiane Radzimski; Jonas Schmidt-Chanasit; Petra Emmerich; Marjan van Esbroeck; Janke Schinkel; Martin P Grobusch; Abraham Goorhuis; Jens M Warnecke; Erik Lattwein; Lars Komorowski; Andrea Deerberg; Sandra Saschenbrecker; Winfried Stöcker; Wolfgang Schlumberger Journal: Euro Surveill Date: 2016-12-15
Authors: Vidyleison N Camargos; Giselle Foureaux; Daniel C Medeiros; Vivian T da Silveira; Celso M Queiroz-Junior; Ana Luisa B Matosinhos; André F A Figueiredo; Carla D F Sousa; Thaiane P Moreira; Victória F Queiroz; Ana Carolina F Dias; Karina T O Santana; Ingredy Passos; Ana Luíza C V Real; Ludmila C Silva; Flávio A G Mourão; Natália T Wnuk; Milton A P Oliveira; Soraia Macari; Tarcília Silva; Gustavo P Garlet; Joshua A Jackman; Frederico M Soriani; Márcio F D Moraes; Eduardo M A M Mendes; Fabíola M Ribeiro; Guilherme M J Costa; Antônio L Teixeira; Nam-Joon Cho; Antônio C P Oliveira; Mauro M Teixeira; Vivian V Costa; Danielle G Souza Journal: EBioMedicine Date: 2019-05-23 Impact factor: 8.143