Cao Deping1, Jiang Bofan2, Zhang Yaogang3, Pang Mingquan3. 1. Department of Human Parasitology, Guilin Medical College, Guilin, 541101, Guangxi Zhuang Autonomous, China. qhmccdp@163.com.ph. 2. The Department of Pathogenic Biology of Qinghai University Medical College, Xining, 810001, Qinghai Province, China. 3. The Echinococcosis Key Laboratory of Affiliated Hospital of Qinghai University, Xining, 810001, Qinghai Province, China.
Abstract
BACKGROUND: Alveolar echinococcosis (AE) is caused by parasitic infection by Echinococcus multilocularis. Its diagnosis is usually based on clinical symptoms, ultrasound, and other imaging methods. MicroRNAs (miRNAs) play important roles in disease processes and can exist in a highly stable cell-free form in body fluids. It is important to identify specific, sensitive diagnostic markers for early diagnosis and evaluation of AE. In this study, we examined hsa-miR-125b-5p as a potential plasma biomarker of E. multilocularis infection. METHODS: Plasma samples from patients with AE and healthy individuals were screened for the presence of five miRNAs using miRNA chips. We used quantitative polymerase chain reaction to measure miRNA expression levels in plasma and liver tissue samples from patients with AE. RESULTS: hsa-miR-125b-5p was stably upregulated in the plasma and liver tissue samples from patients with AE. CONCLUSIONS: The results suggest that hsa-miR-125b-5p may be a promising biomarker for early, non-invasive diagnosis of AE.
BACKGROUND:Alveolar echinococcosis (AE) is caused by parasitic infection by Echinococcus multilocularis. Its diagnosis is usually based on clinical symptoms, ultrasound, and other imaging methods. MicroRNAs (miRNAs) play important roles in disease processes and can exist in a highly stable cell-free form in body fluids. It is important to identify specific, sensitive diagnostic markers for early diagnosis and evaluation of AE. In this study, we examined hsa-miR-125b-5p as a potential plasma biomarker of E. multilocularisinfection. METHODS: Plasma samples from patients with AE and healthy individuals were screened for the presence of five miRNAs using miRNA chips. We used quantitative polymerase chain reaction to measure miRNA expression levels in plasma and liver tissue samples from patients with AE. RESULTS:hsa-miR-125b-5p was stably upregulated in the plasma and liver tissue samples from patients with AE. CONCLUSIONS: The results suggest that hsa-miR-125b-5p may be a promising biomarker for early, non-invasive diagnosis of AE.
Authors: Patrick Giraudoux; David Pleydell; Francis Raoul; Jean-Pierre Quéré; Qian Wang; Yurong Yang; Dominique A Vuitton; Jiamen Qiu; Wen Yang; Philip S Craig Journal: Parasitol Int Date: 2005-12-19 Impact factor: 2.230
Authors: F Li; P Zhou; W Deng; J Wang; R Mao; Y Zhang; J Li; J Yu; F Yang; Y Huang; M Lu; J Zhang Journal: Clin Microbiol Infect Date: 2016-01-21 Impact factor: 8.067
Authors: Samir Kelada; Praveen Sethupathy; Isobel S Okoye; Eleni Kistasis; Stephanie Czieso; Sandra D White; David Chou; Craig Martens; Stacy M Ricklefs; Kimmo Virtaneva; Dan E Sturdevant; Stephen F Porcella; Yasmine Belkaid; Thomas A Wynn; Mark S Wilson Journal: PLoS Pathog Date: 2013-06-27 Impact factor: 6.823
Authors: Yi Mu; Pengfei Cai; Remigio M Olveda; Allen G Ross; David U Olveda; Donald P McManus Journal: Parasitology Date: 2019-12-16 Impact factor: 3.234